Detecting expression patterns of Wnt pathway components in sea urchin embryos

Joanna M. Bince, Chieh Fu Peng, Athula Wikramanayake

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The animal-vegetal (A-V) axis is a maternally established asymmetry that is present in most animal eggs, and it plays an important role in germ-layer segregation. Recent work has shown that the canonical Wnt signaling pathway plays an evolutionarily conserved role in specifying and patterning this axis. However, the precise mechanisms by which this pathway is activated in the early embryo to pattern the A-V axis are not known in most animals. The availability of the Strongylocentrotus purpuratus genome sequence, the ability to experimentally manipulate eggs and early embryos using embryological and molecular tools, and the superior optical clarity of sea urchin embryos makes them an important model for investigating the role of the canonical Wnt pathway in specifying and patterning the A-V axis. Here, we provide detailed protocols for determining the expression and localization of mRNA and proteins in early sea urchin embryos, which can be used in studies examining the regulation of Wnt signaling along the A-V axis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages201-211
Number of pages11
Volume469
DOIs
StatePublished - Dec 1 2008

Publication series

NameMethods in Molecular Biology
Volume469
ISSN (Print)10643745

Fingerprint

Wnt Signaling Pathway
Sea Urchins
Embryonic Structures
Eggs
Strongylocentrotus purpuratus
Germ Layers
Genome
Messenger RNA
Proteins

Keywords

  • Animal-vegetal axis
  • Immunoflourescence
  • Sea urchin
  • Whole-mount in situ hybridization
  • Wnt signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bince, J. M., Peng, C. F., & Wikramanayake, A. (2008). Detecting expression patterns of Wnt pathway components in sea urchin embryos. In Methods in Molecular Biology (Vol. 469, pp. 201-211). (Methods in Molecular Biology; Vol. 469). https://doi.org/10.1007/978-1-60327-469-2_15

Detecting expression patterns of Wnt pathway components in sea urchin embryos. / Bince, Joanna M.; Peng, Chieh Fu; Wikramanayake, Athula.

Methods in Molecular Biology. Vol. 469 2008. p. 201-211 (Methods in Molecular Biology; Vol. 469).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bince, JM, Peng, CF & Wikramanayake, A 2008, Detecting expression patterns of Wnt pathway components in sea urchin embryos. in Methods in Molecular Biology. vol. 469, Methods in Molecular Biology, vol. 469, pp. 201-211. https://doi.org/10.1007/978-1-60327-469-2_15
Bince JM, Peng CF, Wikramanayake A. Detecting expression patterns of Wnt pathway components in sea urchin embryos. In Methods in Molecular Biology. Vol. 469. 2008. p. 201-211. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-469-2_15
Bince, Joanna M. ; Peng, Chieh Fu ; Wikramanayake, Athula. / Detecting expression patterns of Wnt pathway components in sea urchin embryos. Methods in Molecular Biology. Vol. 469 2008. pp. 201-211 (Methods in Molecular Biology).
@inbook{d9269671aa124526ae06948df4c0a08f,
title = "Detecting expression patterns of Wnt pathway components in sea urchin embryos",
abstract = "The animal-vegetal (A-V) axis is a maternally established asymmetry that is present in most animal eggs, and it plays an important role in germ-layer segregation. Recent work has shown that the canonical Wnt signaling pathway plays an evolutionarily conserved role in specifying and patterning this axis. However, the precise mechanisms by which this pathway is activated in the early embryo to pattern the A-V axis are not known in most animals. The availability of the Strongylocentrotus purpuratus genome sequence, the ability to experimentally manipulate eggs and early embryos using embryological and molecular tools, and the superior optical clarity of sea urchin embryos makes them an important model for investigating the role of the canonical Wnt pathway in specifying and patterning the A-V axis. Here, we provide detailed protocols for determining the expression and localization of mRNA and proteins in early sea urchin embryos, which can be used in studies examining the regulation of Wnt signaling along the A-V axis.",
keywords = "Animal-vegetal axis, Immunoflourescence, Sea urchin, Whole-mount in situ hybridization, Wnt signaling",
author = "Bince, {Joanna M.} and Peng, {Chieh Fu} and Athula Wikramanayake",
year = "2008",
month = "12",
day = "1",
doi = "10.1007/978-1-60327-469-2_15",
language = "English",
isbn = "9781603274685",
volume = "469",
series = "Methods in Molecular Biology",
pages = "201--211",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Detecting expression patterns of Wnt pathway components in sea urchin embryos

AU - Bince, Joanna M.

AU - Peng, Chieh Fu

AU - Wikramanayake, Athula

PY - 2008/12/1

Y1 - 2008/12/1

N2 - The animal-vegetal (A-V) axis is a maternally established asymmetry that is present in most animal eggs, and it plays an important role in germ-layer segregation. Recent work has shown that the canonical Wnt signaling pathway plays an evolutionarily conserved role in specifying and patterning this axis. However, the precise mechanisms by which this pathway is activated in the early embryo to pattern the A-V axis are not known in most animals. The availability of the Strongylocentrotus purpuratus genome sequence, the ability to experimentally manipulate eggs and early embryos using embryological and molecular tools, and the superior optical clarity of sea urchin embryos makes them an important model for investigating the role of the canonical Wnt pathway in specifying and patterning the A-V axis. Here, we provide detailed protocols for determining the expression and localization of mRNA and proteins in early sea urchin embryos, which can be used in studies examining the regulation of Wnt signaling along the A-V axis.

AB - The animal-vegetal (A-V) axis is a maternally established asymmetry that is present in most animal eggs, and it plays an important role in germ-layer segregation. Recent work has shown that the canonical Wnt signaling pathway plays an evolutionarily conserved role in specifying and patterning this axis. However, the precise mechanisms by which this pathway is activated in the early embryo to pattern the A-V axis are not known in most animals. The availability of the Strongylocentrotus purpuratus genome sequence, the ability to experimentally manipulate eggs and early embryos using embryological and molecular tools, and the superior optical clarity of sea urchin embryos makes them an important model for investigating the role of the canonical Wnt pathway in specifying and patterning the A-V axis. Here, we provide detailed protocols for determining the expression and localization of mRNA and proteins in early sea urchin embryos, which can be used in studies examining the regulation of Wnt signaling along the A-V axis.

KW - Animal-vegetal axis

KW - Immunoflourescence

KW - Sea urchin

KW - Whole-mount in situ hybridization

KW - Wnt signaling

UR - http://www.scopus.com/inward/record.url?scp=59249095981&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59249095981&partnerID=8YFLogxK

U2 - 10.1007/978-1-60327-469-2_15

DO - 10.1007/978-1-60327-469-2_15

M3 - Chapter

SN - 9781603274685

VL - 469

T3 - Methods in Molecular Biology

SP - 201

EP - 211

BT - Methods in Molecular Biology

ER -