Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos

Shalika Kumburegama, Naveen Wijesena, Athula H. Wikramanayake

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

The anthozoan cnidarian Nematostella vectensis has emerged as a key model system for evolutionary developmental biology studies, and this animal' usefulness will grow with the recent sequencing of its genome. In particular, work done in Nematostella is providing insight into the role of the Wnt pathway in the evolution of pattern formation. This chapter describes methods to maintain and spawn these animals, and detailed protocols to detect expression patterns of Wnt pathway components in Nematostella eggs and embryos.

Original languageEnglish (US)
Title of host publicationWnt Signaling
Subtitle of host publicationVolume 2 Pathway Models
EditorsElizabeth Vincan
Pages55-67
Number of pages13
DOIs
StatePublished - Dec 1 2008

Publication series

NameMethods in Molecular Biology
Volume469
ISSN (Print)1064-3745

Keywords

  • Cnidarian
  • Immuno-cytochemistry
  • Nematostella
  • Whole-mount in situ hybridization
  • Wnt signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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