Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos

Shalika Kumburegama, Naveen Wijesena, Athula Wikramanayake

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

The anthozoan cnidarian Nematostella vectensis has emerged as a key model system for evolutionary developmental biology studies, and this animal' usefulness will grow with the recent sequencing of its genome. In particular, work done in Nematostella is providing insight into the role of the Wnt pathway in the evolution of pattern formation. This chapter describes methods to maintain and spawn these animals, and detailed protocols to detect expression patterns of Wnt pathway components in Nematostella eggs and embryos.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages55-67
Number of pages13
Volume469
DOIs
StatePublished - Dec 1 2008

Publication series

NameMethods in Molecular Biology
Volume469
ISSN (Print)10643745

Fingerprint

Wnt Signaling Pathway
Embryonic Structures
Cnidaria
Developmental Biology
Eggs
Genome

Keywords

  • Cnidarian
  • Immuno-cytochemistry
  • Nematostella
  • Whole-mount in situ hybridization
  • Wnt signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Kumburegama, S., Wijesena, N., & Wikramanayake, A. (2008). Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos. In Methods in Molecular Biology (Vol. 469, pp. 55-67). (Methods in Molecular Biology; Vol. 469). https://doi.org/10.1007/978-1-60327-469-2_6

Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos. / Kumburegama, Shalika; Wijesena, Naveen; Wikramanayake, Athula.

Methods in Molecular Biology. Vol. 469 2008. p. 55-67 (Methods in Molecular Biology; Vol. 469).

Research output: Chapter in Book/Report/Conference proceedingChapter

Kumburegama, S, Wijesena, N & Wikramanayake, A 2008, Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos. in Methods in Molecular Biology. vol. 469, Methods in Molecular Biology, vol. 469, pp. 55-67. https://doi.org/10.1007/978-1-60327-469-2_6
Kumburegama S, Wijesena N, Wikramanayake A. Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos. In Methods in Molecular Biology. Vol. 469. 2008. p. 55-67. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-60327-469-2_6
Kumburegama, Shalika ; Wijesena, Naveen ; Wikramanayake, Athula. / Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos. Methods in Molecular Biology. Vol. 469 2008. pp. 55-67 (Methods in Molecular Biology).
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