Desmopressin (DDAVP) acts on platelets to generate platelet microparticles and enhanced procoagulant activity

Lawrence L. Horstman, Benjamin J. Valle-Riestra, Wenche Jy, Fang Wang, Wei Mao, Yeon S. Ahn

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Desmopressin (DDAVP), an analog of vasopressin (AVP), has wide clinical application as an anti-hemorrhagic (AH) agent. DDAVP in vivo releases vWF from endothelial cells but is reported to have little action on platelets. However, DDAVP is often used to improve hemostasis in platelet dysfunctions. We examined the effect of DDAVP on platelet microparticle (PMP) formation and procoagulant activity in vitro using platelets from normal volunteers and in vivo in six patients receiving DDAVP therapy. In the former, platelets were incubated with DDAVP (0.5 to 25 nM) and PMP released were stained with FITC-labeled MAb α-GP IIb IIIa for flow cytometry. Procoagulant activity was measured in a clot-based assay using Russel's viper venom (RVV) calibrated with cephalin. A mean increase of 2-3 fold was observed in both PMP and procoagulant activity. Parallel to these observations was a dose-dependent rise in organelle-associated Ca2+. The assays were also performed on six patients prior to and at one hour after infusion of DDAVP, and similar but lesser effects were observed. We conclude that DDAVP acts on platelets in vitro, and that these effects may contribute to the hemostatic action of DDAVP in platelet dysfunctions in vivo.

Original languageEnglish (US)
Pages (from-to)163-174
Number of pages12
JournalThrombosis Research
Volume79
Issue number2
DOIs
StatePublished - Jul 15 1995

Keywords

  • DDAVP/desmopressin
  • hemostasis
  • platelet microparticles

ASJC Scopus subject areas

  • Hematology
  • Cardiology and Cardiovascular Medicine

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