Design of a membrane fluorescent sensor based on photo-cross-linked PEG hydrogel

Yujun Zheng, Kerim M. Gattás-Asfura, Changqing Li, Fotios M. Andreopoulos, Si M. Pham, Roger M. Leblanc

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

A polymeric hydrogel film was made photochemically for the fluorescent detection of copper ions. An eight-branched PEG macromer having a nitrocinnamate (NC) moiety as the pendant group was synthesized and found to form a gel upon exposure to 365-nm irradiation in the absence of any photoinitiators or catalysts. Peptide 1 (Dns-Gly-His-Lys(NC)) was synthesized with Fmoc solid-phase method and contained three essential parts: a dansyl fluorophore for fluorescent signal transduction, a metal binding site for selective recognition of copper ions from aqueous solution, and a NC group for interaction with PEG-NC macromer. The peptide was found to be efficiently immobilized within the hydrogel network during the photo-cross-linking process. The prepared PEG-NC/1 gel film is strongly fluorescent and can be quenched by the presence of copper ions. The quenching process was reversed by acid treatment and an excellent reversibility was obtained. The designed membrane sensor showed a superior selectivity toward copper ions as demonstrated by fluorescence spectroscopy and epifluorescence imaging.

Original languageEnglish (US)
Pages (from-to)483-488
Number of pages6
JournalJournal of Physical Chemistry B
Volume107
Issue number2
DOIs
StatePublished - Jan 16 2003

    Fingerprint

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this