Design, fabrication, and characterization of a composite scaffold for bone tissue engineering

Federica Boschetti, Alice Tomei, S. Turri, M. A. Swartz, M. Levi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Poly(lactide-co-glycolide) (PLGA) scaffolds have been successfully used in bone tissue engineering, with or without hydroxyapatite (HA) and with a macroporosity given either by simple PLGA sphere packaging and/or by leaching out NaCI. The objective of this work was the optimization of the design parameters for bone tissue engineering scaffolds made by sintering microspheres of PLGA, HA nanocrystals for matrix reinforcement and osteoconduction, and salt crystals for macroporosity and control of matrix pore size. Microsphere fabrication by a single-emulsion and solvent evaporation technique was first optimized to obtain a high yield of PLGA microspheres with a diameter between 80 and 300 μm. The influence of the sintering process and matrix composition on the scaffold structure was then evaluated morphologically and mechanically. Three scaffold types were tested for biocompatibility by culturing with human fibroblasts for up to 14 days. The most important parameters to obtain microspheres with the selected diameter range were the viscosity ratio of the dispersed phase to the continuous phase and the relative volume fraction of the 2 phases. The Young's modulus and the ultimate strength of the sintered matrices ranged between 168-265 MPa and 6-17 MPa, respectively, within the range for trabecular bone. Biocompatibility was demonstrated by fibroblast adhesion, proliferation, and spreading throughout the matrix. This work builds upon previous work of the PLGA/HA sintering technique to give design criteria for fabricating a bone tissue engineered matrix with optimized morphological, functional, and biological properties to fit the requirements of bone replacements.

Original languageEnglish
Pages (from-to)697-707
Number of pages11
JournalInternational Journal of Artificial Organs
Volume31
Issue number8
StatePublished - Aug 1 2008
Externally publishedYes

Fingerprint

Tissue Engineering
Scaffolds (biology)
Tissue engineering
Bone
Microspheres
Bone and Bones
Fabrication
Durapatite
Composite materials
Hydroxyapatite
Sintering
Fibroblasts
Biocompatibility
Tissue Scaffolds
Polyglactin 910
Bone Regeneration
Elastic Modulus
Product Packaging
Emulsions
Viscosity

Keywords

  • Bone scaffold
  • Design parameters
  • Mechanical characterization

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Design, fabrication, and characterization of a composite scaffold for bone tissue engineering. / Boschetti, Federica; Tomei, Alice; Turri, S.; Swartz, M. A.; Levi, M.

In: International Journal of Artificial Organs, Vol. 31, No. 8, 01.08.2008, p. 697-707.

Research output: Contribution to journalArticle

Boschetti, Federica ; Tomei, Alice ; Turri, S. ; Swartz, M. A. ; Levi, M. / Design, fabrication, and characterization of a composite scaffold for bone tissue engineering. In: International Journal of Artificial Organs. 2008 ; Vol. 31, No. 8. pp. 697-707.
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