Deregulation of epidermal stem cell niche contributes to pathogenesis of nonhealing venous ulcers

Olivera Stojadinovic, Irena Pastar, Aron G. Nusbaum, Sasa Vukelic, Agata Krzyzanowska, Marjana Tomic-Canic

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The epidermis is maintained by epidermal stem cells (ESCs) that reside in distinct niches and contribute to homeostasis and wound closure. Keratinocytes at the nonhealing edges of venous ulcers (VUs) are healing-incompetent, hyperproliferative, and nonmigratory, suggesting deregulation of ESCs. To date, genes which regulate ESC niches have been studied in mice only. Utilizing microarray analysis of VU nonhealing edges, we identified changes in expression of genes harboring regulation of ESCs and their fate. In a prospective clinical study of 10 VUs, we confirmed suppression of the bone morphogenetic protein receptor (BMPR) and GATA binding protein 3 (GATA3) as well as inhibitors of DNA-binding proteins 2 and 4 (ID2 and ID4). We also found decreased levels of phosphorylated glycogen synthase kinase 3 (GSK3), nuclear presence of β-catenin, and overexpression of its transcriptional target, c-myc, indicating activation of the Wnt pathway. Additionally, we found down-regulation of leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1), a gene important for maintaining ESCs in a quiescent state, and absence of keratin 15 (K15), a marker of the basal stem cell compartment suggesting local depletion of ESCs. Our study shows that loss of genes important for regulation of ESCs and their fate along with activation of β-catenin and c-myc in the VU may contribute to ESC deprivation and a hyperproliferative, nonmigratory healing incapable wound edge.

Original languageEnglish
Pages (from-to)220-227
Number of pages8
JournalWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society
Volume22
Issue number2
DOIs
StatePublished - Jan 1 2014

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Stem Cell Niche
Varicose Ulcer
Stem Cells
Catenins
Inhibitor of Differentiation Protein 2
Keratin-15
Bone Morphogenetic Protein Receptors
Genes
Glycogen Synthase Kinase 3
Wnt Signaling Pathway
Gene Expression Regulation
Microarray Analysis
Keratinocytes
Epidermis
Leucine
Wound Healing
Carrier Proteins
Homeostasis
Down-Regulation
Prospective Studies

ASJC Scopus subject areas

  • Medicine(all)

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Deregulation of epidermal stem cell niche contributes to pathogenesis of nonhealing venous ulcers. / Stojadinovic, Olivera; Pastar, Irena; Nusbaum, Aron G.; Vukelic, Sasa; Krzyzanowska, Agata; Tomic-Canic, Marjana.

In: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, Vol. 22, No. 2, 01.01.2014, p. 220-227.

Research output: Contribution to journalArticle

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