Derangements of post-ischemic cerebral blood flow by protein kinase C delta

Hung Wen Lin, R. A. DeFazio, David Della Morte, J. W. Thompson, S. V. Narayanan, Ami Raval, I. Saul, Kunjan R Dave, Miguel Perez-Pinzon

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cerebral ischemia causes blood flow derangements characterized by hyperemia (increased cerebral blood flow, CBF) and subsequent hypoperfusion (decreased CBF). We previously demonstrated that protein kinase C delta (δPKC) plays an important role in hippocampal neuronal death after ischemia. However, whether part of this protection is due to the role of δPKC on CBF following cerebral ischemia remains poorly understood. We hypothesized that δPKC exacerbates hyperemia and subsequent hypoperfusion resulting in CBF derangements following ischemia. Sprague-Dawley (SD) rats pretreated with a δPKC specific inhibitor (δV1-1, 0.5 mg/kg) exhibited attenuation of hyperemia and latent hypoperfusion characterized by vasoconstriction followed by vasodilation of microvessels after 2-vessel occlusion plus hypotension measured by 2-photon microscopy. In an asphyxial cardiac arrest model (ACA), SD rats treated with δV1-1 (pre- and post-ischemia) exhibited improved perfusion after 24 h and less hippocampal CA1 neuronal death 7 days after ACA. These results suggest possible therapeutic potential of δPKC in modulating CBF and neuronal damage after cerebral ischemia.

Original languageEnglish
Pages (from-to)566-576
Number of pages11
JournalNeuroscience
Volume171
Issue number2
DOIs
StatePublished - Dec 1 2010

Fingerprint

Cerebrovascular Circulation
Protein Kinase C-delta
Blood Proteins
Hyperemia
Ischemia
Brain Ischemia
Heart Arrest
Sprague Dawley Rats
Microvessels
Vasoconstriction
Photons
Vasodilation
Hypotension
Microscopy
Perfusion

Keywords

  • Asphyxial cardiac arrest
  • Cerebral ischemia
  • Neuroprotection
  • Protein kinase C delta
  • Two-photon microscopy
  • Two-vessel occlusion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Derangements of post-ischemic cerebral blood flow by protein kinase C delta. / Wen Lin, Hung; DeFazio, R. A.; Della Morte, David; Thompson, J. W.; Narayanan, S. V.; Raval, Ami; Saul, I.; Dave, Kunjan R; Perez-Pinzon, Miguel.

In: Neuroscience, Vol. 171, No. 2, 01.12.2010, p. 566-576.

Research output: Contribution to journalArticle

Wen Lin, Hung ; DeFazio, R. A. ; Della Morte, David ; Thompson, J. W. ; Narayanan, S. V. ; Raval, Ami ; Saul, I. ; Dave, Kunjan R ; Perez-Pinzon, Miguel. / Derangements of post-ischemic cerebral blood flow by protein kinase C delta. In: Neuroscience. 2010 ; Vol. 171, No. 2. pp. 566-576.
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