Depletion of muscle mitochondrial DNA in AIDS patients with zidovudine-induced myopathy

E. Arnaudo, S. Shanske, S. DiMauro, E. A. Schon, C. T. Moraes, E. A. Schon, M. Dalakas

Research output: Contribution to journalArticlepeer-review

446 Scopus citations

Abstract

Long-term zidovudine therapy in patients with human immunodeficiency virus (HIV) infection can cause a destructive mitochondrial myopathy with histological features of ragged-red fibres (RRF) and proliferation of abnormal mitochondria. In 9 zidovudine-treated patients with this myopathy we found severely reduced amounts (up to 78% reduction vs normal adult controls) of mitochondrial DNA (mtDNA) in muscle biopsy specimens by means of Southern blotting. In 2 HIV-positive patients who had not received zidovudine, muscle mtDNA content did not differ from that in the 4 controls. Depletion of mtD NA seems to be reversible, since 1 patient showed a substantial reduction in RRF and a concomitant pronounced increase in muscle mtDNA content after zidovudine therapy was discontinued. Depletion of muscle mtDNA is probably due to zidovudine-induced inhibition of mtDNA replication by DNA polymerase gamma and is not a secondary effect of HIV infection.

Original languageEnglish (US)
Pages (from-to)508-510
Number of pages3
JournalThe Lancet
Volume337
Issue number8740
DOIs
StatePublished - Mar 2 1991
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Depletion of muscle mitochondrial DNA in AIDS patients with zidovudine-induced myopathy'. Together they form a unique fingerprint.

Cite this