Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli

Gerard G. Bouffard, Kenneth E. Rudd, Sankar L. Adhya

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

A new gene (galM) has been identified as the fourth cistron of the gal operon, encoding enzymes for the metabolism of galactose and lactose in Escherichia coli. Induction of the gal operon either from the gal promoters or from a neighboring prophage λ promoter expresses the galM gene as well. The new structure of the gal operon from the promoter end is galE-galT-galK-galM in counter-clockwise orientation on the chromosome. Genetic and biochemical analyses have revealed that the galM gene product has mutarotase activity, which converts α-aldose to the β-anomer. Unlike mutarotase from other bacteria in which the enzyme is primarily processed for export and secretion, the mutarotase from E. coli does not appear to be processed and yet is still found in periplasm (and culture media when overexpressed) in significant amounts. Although the interconversion of the sugar anomers occurs spontaneously in pure water in vitro, the in vivo formation of α-D-galactopyranose (the substrate for phosphorylation) from β-D-galactopyranose (generated by β-galactosidase hydrolysis of lactose) is largely dependent upon the presence of the mutarotase. This shows that efficient lactose metabolism recquires mutarotase. These results give credence to the idea that the activity of intracellular water is not high enough to permit a simple extrapolation of observed in vitro reactions to in vivo situations in every case.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalJournal of Molecular Biology
Volume244
Issue number3
DOIs
StatePublished - Dec 14 1994
Externally publishedYes

Fingerprint

Lactose
Operon
Escherichia coli
Galactose
Genes
Galactosidases
Prophages
Periplasm
Water
Enzymes
Culture Media
Molecular Biology
Hydrolysis
Chromosomes
Phosphorylation
aldose 1-epimerase
Bacteria
In Vitro Techniques

Keywords

  • β-D-galactoside
  • Aldose-1-epimerase
  • Galactose
  • GalM
  • Glucose

ASJC Scopus subject areas

  • Virology

Cite this

Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli. / Bouffard, Gerard G.; Rudd, Kenneth E.; Adhya, Sankar L.

In: Journal of Molecular Biology, Vol. 244, No. 3, 14.12.1994, p. 269-278.

Research output: Contribution to journalArticle

Bouffard, Gerard G. ; Rudd, Kenneth E. ; Adhya, Sankar L. / Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli. In: Journal of Molecular Biology. 1994 ; Vol. 244, No. 3. pp. 269-278.
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