Deoxyribonuclease induction in apoptotic cytotoxic T lymphocytes

Ge Deng, Eckhard R. Podack

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

IL-2-dependent CTLL2 cells, upon IL-2 deprivation, die by apoptosis, which is accompanied by the fragmentation of genomic DNA. Two major deoxyribonuclease activities were detected in the extract of IL-2-deprived CTLL2 cells in a zymographic assay. They were designated nuc-58 and nuc-40, based on their apparent molecular mass of 58 and 40 kDa. The activity of both DNases was greatly induced in CTLL2 cells deprived of IL-2 or treated with the kinase inhibitor staurosporine. Deregulated expression of bcl-2 cDNA suppressed the induction of both nuclease activities. Nuc-58 was dependent on both Ca2+ and Mg2+ ions whereas nuc-40 was also active in the presence of Mg2+ ions alone. Nuc-40 showed a preferential nuclear localization over that of nuc-58, which was found primarily in the cytoplasm. Optimal activity of both DNases required neutral pH and was inhibited by zinc ions. The physicochemical characteristics of the nucleases indicate that they are novel DNases associated with apoptosis in CTLL2 cells.

Original languageEnglish
Pages (from-to)665-669
Number of pages5
JournalFASEB Journal
Volume9
Issue number9
StatePublished - Dec 1 1995
Externally publishedYes

Fingerprint

deoxyribonucleases
cytotoxic T-lymphocytes
T-cells
Deoxyribonucleases
Cytotoxic T-Lymphocytes
interleukin-2
Interleukin-2
nucleases
Ions
ions
apoptosis
cells
Apoptosis
Staurosporine
Molecular mass
DNA Fragmentation
Zinc
Assays
Cytoplasm
phosphotransferases (kinases)

Keywords

  • Apoptosis
  • Cell death
  • DNA fragmentation
  • Growth factor deprivation
  • Nuclease

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Deng, G., & Podack, E. R. (1995). Deoxyribonuclease induction in apoptotic cytotoxic T lymphocytes. FASEB Journal, 9(9), 665-669.

Deoxyribonuclease induction in apoptotic cytotoxic T lymphocytes. / Deng, Ge; Podack, Eckhard R.

In: FASEB Journal, Vol. 9, No. 9, 01.12.1995, p. 665-669.

Research output: Contribution to journalArticle

Deng, G & Podack, ER 1995, 'Deoxyribonuclease induction in apoptotic cytotoxic T lymphocytes', FASEB Journal, vol. 9, no. 9, pp. 665-669.
Deng, Ge ; Podack, Eckhard R. / Deoxyribonuclease induction in apoptotic cytotoxic T lymphocytes. In: FASEB Journal. 1995 ; Vol. 9, No. 9. pp. 665-669.
@article{619ea2cc7ab046799b6d5e277f9d510f,
title = "Deoxyribonuclease induction in apoptotic cytotoxic T lymphocytes",
abstract = "IL-2-dependent CTLL2 cells, upon IL-2 deprivation, die by apoptosis, which is accompanied by the fragmentation of genomic DNA. Two major deoxyribonuclease activities were detected in the extract of IL-2-deprived CTLL2 cells in a zymographic assay. They were designated nuc-58 and nuc-40, based on their apparent molecular mass of 58 and 40 kDa. The activity of both DNases was greatly induced in CTLL2 cells deprived of IL-2 or treated with the kinase inhibitor staurosporine. Deregulated expression of bcl-2 cDNA suppressed the induction of both nuclease activities. Nuc-58 was dependent on both Ca2+ and Mg2+ ions whereas nuc-40 was also active in the presence of Mg2+ ions alone. Nuc-40 showed a preferential nuclear localization over that of nuc-58, which was found primarily in the cytoplasm. Optimal activity of both DNases required neutral pH and was inhibited by zinc ions. The physicochemical characteristics of the nucleases indicate that they are novel DNases associated with apoptosis in CTLL2 cells.",
keywords = "Apoptosis, Cell death, DNA fragmentation, Growth factor deprivation, Nuclease",
author = "Ge Deng and Podack, {Eckhard R.}",
year = "1995",
month = "12",
day = "1",
language = "English",
volume = "9",
pages = "665--669",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "9",

}

TY - JOUR

T1 - Deoxyribonuclease induction in apoptotic cytotoxic T lymphocytes

AU - Deng, Ge

AU - Podack, Eckhard R.

PY - 1995/12/1

Y1 - 1995/12/1

N2 - IL-2-dependent CTLL2 cells, upon IL-2 deprivation, die by apoptosis, which is accompanied by the fragmentation of genomic DNA. Two major deoxyribonuclease activities were detected in the extract of IL-2-deprived CTLL2 cells in a zymographic assay. They were designated nuc-58 and nuc-40, based on their apparent molecular mass of 58 and 40 kDa. The activity of both DNases was greatly induced in CTLL2 cells deprived of IL-2 or treated with the kinase inhibitor staurosporine. Deregulated expression of bcl-2 cDNA suppressed the induction of both nuclease activities. Nuc-58 was dependent on both Ca2+ and Mg2+ ions whereas nuc-40 was also active in the presence of Mg2+ ions alone. Nuc-40 showed a preferential nuclear localization over that of nuc-58, which was found primarily in the cytoplasm. Optimal activity of both DNases required neutral pH and was inhibited by zinc ions. The physicochemical characteristics of the nucleases indicate that they are novel DNases associated with apoptosis in CTLL2 cells.

AB - IL-2-dependent CTLL2 cells, upon IL-2 deprivation, die by apoptosis, which is accompanied by the fragmentation of genomic DNA. Two major deoxyribonuclease activities were detected in the extract of IL-2-deprived CTLL2 cells in a zymographic assay. They were designated nuc-58 and nuc-40, based on their apparent molecular mass of 58 and 40 kDa. The activity of both DNases was greatly induced in CTLL2 cells deprived of IL-2 or treated with the kinase inhibitor staurosporine. Deregulated expression of bcl-2 cDNA suppressed the induction of both nuclease activities. Nuc-58 was dependent on both Ca2+ and Mg2+ ions whereas nuc-40 was also active in the presence of Mg2+ ions alone. Nuc-40 showed a preferential nuclear localization over that of nuc-58, which was found primarily in the cytoplasm. Optimal activity of both DNases required neutral pH and was inhibited by zinc ions. The physicochemical characteristics of the nucleases indicate that they are novel DNases associated with apoptosis in CTLL2 cells.

KW - Apoptosis

KW - Cell death

KW - DNA fragmentation

KW - Growth factor deprivation

KW - Nuclease

UR - http://www.scopus.com/inward/record.url?scp=0029036047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029036047&partnerID=8YFLogxK

M3 - Article

C2 - 7768359

AN - SCOPUS:0029036047

VL - 9

SP - 665

EP - 669

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 9

ER -