Dense hydroxylation (HA) and microporous HA resorption

Andrw J. Tofe, Barbara A. Watson, Herman S Cheung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this presentation, the density and porosity of commercially available hydroxylapatite (HA) bone grafting materials analyzed by scanning electron microscopy (SEM). The calcium phosphate phases and molecular constituents responsible for dissolution/ resorption of hydroxylapatite (HA) were analyzed by x-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy. The lack of microporosity explains the nonresorptive nature of truly dense HA while the presence of microporosity leads to cell-mediated resorption. The latter was evaluated using the in vitro cell culture model with human fibroblasts. Scanning electron and light microscopy confirmed the endocytosis mechanism for porous HA and illustrated the inhibition of cellular invasion/resorption by dense HA. A multi-analytical /spectroscopic approach coupled to in vitro cell culture studies provides insight into the dissolution/resorption of HA grafting materials and HA coating on implants.

Original languageEnglish
Title of host publicationProceedings of the Symposium on Characterization and Performance of Calcium Phosphate Coatings for Implants
EditorsEmanuel Horowitz, Jack E. Parr
Place of PublicationPhiladelphia, PA, United States
PublisherPubl by ASTM
Pages163-169
Number of pages7
ISBN (Print)0803118546
StatePublished - Dec 1 1994
Externally publishedYes
EventProceedings of the Symposium on Characterization and Performance of Calcium Phoshate Coatings for Implants - Miami, FL, USA
Duration: Nov 17 1992Nov 17 1992

Other

OtherProceedings of the Symposium on Characterization and Performance of Calcium Phoshate Coatings for Implants
CityMiami, FL, USA
Period11/17/9211/17/92

Fingerprint

Hydroxylation
Microporosity
Cell culture
Dissolution
Scanning electron microscopy
Calcium phosphate
Fibroblasts
Density (specific gravity)
Optical microscopy
Fourier transform infrared spectroscopy
Bone
Porosity
Diffraction
X rays
Coatings

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Tofe, A. J., Watson, B. A., & Cheung, H. S. (1994). Dense hydroxylation (HA) and microporous HA resorption. In E. Horowitz, & J. E. Parr (Eds.), Proceedings of the Symposium on Characterization and Performance of Calcium Phosphate Coatings for Implants (pp. 163-169). Philadelphia, PA, United States: Publ by ASTM.

Dense hydroxylation (HA) and microporous HA resorption. / Tofe, Andrw J.; Watson, Barbara A.; Cheung, Herman S.

Proceedings of the Symposium on Characterization and Performance of Calcium Phosphate Coatings for Implants. ed. / Emanuel Horowitz; Jack E. Parr. Philadelphia, PA, United States : Publ by ASTM, 1994. p. 163-169.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tofe, AJ, Watson, BA & Cheung, HS 1994, Dense hydroxylation (HA) and microporous HA resorption. in E Horowitz & JE Parr (eds), Proceedings of the Symposium on Characterization and Performance of Calcium Phosphate Coatings for Implants. Publ by ASTM, Philadelphia, PA, United States, pp. 163-169, Proceedings of the Symposium on Characterization and Performance of Calcium Phoshate Coatings for Implants, Miami, FL, USA, 11/17/92.
Tofe AJ, Watson BA, Cheung HS. Dense hydroxylation (HA) and microporous HA resorption. In Horowitz E, Parr JE, editors, Proceedings of the Symposium on Characterization and Performance of Calcium Phosphate Coatings for Implants. Philadelphia, PA, United States: Publ by ASTM. 1994. p. 163-169
Tofe, Andrw J. ; Watson, Barbara A. ; Cheung, Herman S. / Dense hydroxylation (HA) and microporous HA resorption. Proceedings of the Symposium on Characterization and Performance of Calcium Phosphate Coatings for Implants. editor / Emanuel Horowitz ; Jack E. Parr. Philadelphia, PA, United States : Publ by ASTM, 1994. pp. 163-169
@inproceedings{e6b3dd98134840058b1c28e27c134e43,
title = "Dense hydroxylation (HA) and microporous HA resorption",
abstract = "In this presentation, the density and porosity of commercially available hydroxylapatite (HA) bone grafting materials analyzed by scanning electron microscopy (SEM). The calcium phosphate phases and molecular constituents responsible for dissolution/ resorption of hydroxylapatite (HA) were analyzed by x-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy. The lack of microporosity explains the nonresorptive nature of truly dense HA while the presence of microporosity leads to cell-mediated resorption. The latter was evaluated using the in vitro cell culture model with human fibroblasts. Scanning electron and light microscopy confirmed the endocytosis mechanism for porous HA and illustrated the inhibition of cellular invasion/resorption by dense HA. A multi-analytical /spectroscopic approach coupled to in vitro cell culture studies provides insight into the dissolution/resorption of HA grafting materials and HA coating on implants.",
author = "Tofe, {Andrw J.} and Watson, {Barbara A.} and Cheung, {Herman S}",
year = "1994",
month = "12",
day = "1",
language = "English",
isbn = "0803118546",
pages = "163--169",
editor = "Emanuel Horowitz and Parr, {Jack E.}",
booktitle = "Proceedings of the Symposium on Characterization and Performance of Calcium Phosphate Coatings for Implants",
publisher = "Publ by ASTM",

}

TY - GEN

T1 - Dense hydroxylation (HA) and microporous HA resorption

AU - Tofe, Andrw J.

AU - Watson, Barbara A.

AU - Cheung, Herman S

PY - 1994/12/1

Y1 - 1994/12/1

N2 - In this presentation, the density and porosity of commercially available hydroxylapatite (HA) bone grafting materials analyzed by scanning electron microscopy (SEM). The calcium phosphate phases and molecular constituents responsible for dissolution/ resorption of hydroxylapatite (HA) were analyzed by x-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy. The lack of microporosity explains the nonresorptive nature of truly dense HA while the presence of microporosity leads to cell-mediated resorption. The latter was evaluated using the in vitro cell culture model with human fibroblasts. Scanning electron and light microscopy confirmed the endocytosis mechanism for porous HA and illustrated the inhibition of cellular invasion/resorption by dense HA. A multi-analytical /spectroscopic approach coupled to in vitro cell culture studies provides insight into the dissolution/resorption of HA grafting materials and HA coating on implants.

AB - In this presentation, the density and porosity of commercially available hydroxylapatite (HA) bone grafting materials analyzed by scanning electron microscopy (SEM). The calcium phosphate phases and molecular constituents responsible for dissolution/ resorption of hydroxylapatite (HA) were analyzed by x-ray diffraction (XRD) and Fourier Transform Infrared (FTIR) spectroscopy. The lack of microporosity explains the nonresorptive nature of truly dense HA while the presence of microporosity leads to cell-mediated resorption. The latter was evaluated using the in vitro cell culture model with human fibroblasts. Scanning electron and light microscopy confirmed the endocytosis mechanism for porous HA and illustrated the inhibition of cellular invasion/resorption by dense HA. A multi-analytical /spectroscopic approach coupled to in vitro cell culture studies provides insight into the dissolution/resorption of HA grafting materials and HA coating on implants.

UR - http://www.scopus.com/inward/record.url?scp=0028572969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028572969&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0028572969

SN - 0803118546

SP - 163

EP - 169

BT - Proceedings of the Symposium on Characterization and Performance of Calcium Phosphate Coatings for Implants

A2 - Horowitz, Emanuel

A2 - Parr, Jack E.

PB - Publ by ASTM

CY - Philadelphia, PA, United States

ER -