Dengue-2 virus infection of human bone marrow: Characterization of dengue-2 antigen-positive stromal cells

Stephen W. Rothwell, Robert Putnak, Vincent F L A Russa

Research output: Contribution to journalArticle

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Abstract

Dengue is often associated with neutropenia and thrombocytopenia, suggesting that cells of the bone marrow may be targets of dengue viral infections. In this study we infected long-term marrow cultures with dengue type-2 (DEN-2) virus and characterized the viral antigen-positive cells. Using immunofluorescence microscopy and immunohistochemical staining, we demonstrated two types of stromal cells that were positive for DEN-2 virus antigens. The first was a population of relatively small (approximately 25 μM) CD11b/CD18 (MAC-1)-positive cells. When stained with anti-DEN-2 polyclonal antibody, these cells showed viral antigen-positive inclusions and, when stained with anti-tubulin or anti-vimentin antibodies, they showed a diffuse pattern of fluorescence, consistent with mobile dendritic cells with phagocytic functions. The second population of DEN-2 antigen-positive cells comprised a smaller proportion of the total cells. It was made up of larger cells (> 100 μm) that had a well-formed cytoskeletal system as demonstrated by intense staining with anti-actin, anti-tubulin, and anti-vimentin antibodies. When stained with anti-DEN-2 antibody, these cells showed a more diffuse pattern of fluorescence in the perinuclear and Golgi regions, consistent with ongoing virus replication. These large, strongly adherent cells were positive for nerve growth factor receptor, consistent with their identification as adventitial reticular cells. The molecule that mediates the virus interaction with susceptible cells has not previously been identified. Using plasma membrane proteins isolated from K562 cells, virus-binding studies suggest that an approximately 100-kD membrane protein may be involved in the initial virus-cell interaction.

Original languageEnglish
Pages (from-to)503-510
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume54
Issue number5
StatePublished - May 1 1996

Fingerprint

Dengue Virus
Dengue
Virus Diseases
Stromal Cells
Bone Marrow
Antigens
Viral Antigens
Vimentin
Tubulin
Anti-Idiotypic Antibodies
Membrane Proteins
Fluorescence
Staining and Labeling
Viruses
Virus Attachment
Nerve Growth Factor Receptor
Adventitia
K562 Cells
Antibodies
Virus Replication

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Dengue-2 virus infection of human bone marrow : Characterization of dengue-2 antigen-positive stromal cells. / Rothwell, Stephen W.; Putnak, Robert; Russa, Vincent F L A.

In: American Journal of Tropical Medicine and Hygiene, Vol. 54, No. 5, 01.05.1996, p. 503-510.

Research output: Contribution to journalArticle

Rothwell, Stephen W. ; Putnak, Robert ; Russa, Vincent F L A. / Dengue-2 virus infection of human bone marrow : Characterization of dengue-2 antigen-positive stromal cells. In: American Journal of Tropical Medicine and Hygiene. 1996 ; Vol. 54, No. 5. pp. 503-510.
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