Abstract
The initiation of primary immune responses is the key function of specialized antigen presenting cells, the dendritic cells (DC). DC of myeloid origin capture antigens in tissues, migrate to lymphoid organs and stimulate T cell responses. A subset of DC has been described which expresses lymphoid determinants and has potential regulatory functions. Conditional transformation of chicken bone marrow progenitors with v-relER, a v-rel estrogen receptor (ER) fusion gene, allows expansion of progenitors that can be induced to differentiate into DC in vitro. In this paper we describe that v-relER cells exhibit both myeloid and lymphoid surface markers, while B cell, T cell and NK (natural killer)-specific surface markers are absent. v-relER DC express, however, cytoplasmic CD3 protein and mRNA for CD8α and the lymphoid transcription factor GATA-3. These data suggest that v-relER DC might be related to the lymphoid subset of DC described in mammals.
| Original language | English |
|---|---|
| Pages (from-to) | 394-407 |
| Number of pages | 14 |
| Journal | Immunobiology |
| Volume | 202 |
| Issue number | 4 |
| State | Published - Dec 7 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes. / Madruga, J.; Briegel, Karoline; Diebold, S.; Boehmelt, G.; Vogel, F.; Zenke, M.
In: Immunobiology, Vol. 202, No. 4, 07.12.2000, p. 394-407.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes
AU - Madruga, J.
AU - Briegel, Karoline
AU - Diebold, S.
AU - Boehmelt, G.
AU - Vogel, F.
AU - Zenke, M.
PY - 2000/12/7
Y1 - 2000/12/7
N2 - The initiation of primary immune responses is the key function of specialized antigen presenting cells, the dendritic cells (DC). DC of myeloid origin capture antigens in tissues, migrate to lymphoid organs and stimulate T cell responses. A subset of DC has been described which expresses lymphoid determinants and has potential regulatory functions. Conditional transformation of chicken bone marrow progenitors with v-relER, a v-rel estrogen receptor (ER) fusion gene, allows expansion of progenitors that can be induced to differentiate into DC in vitro. In this paper we describe that v-relER cells exhibit both myeloid and lymphoid surface markers, while B cell, T cell and NK (natural killer)-specific surface markers are absent. v-relER DC express, however, cytoplasmic CD3 protein and mRNA for CD8α and the lymphoid transcription factor GATA-3. These data suggest that v-relER DC might be related to the lymphoid subset of DC described in mammals.
AB - The initiation of primary immune responses is the key function of specialized antigen presenting cells, the dendritic cells (DC). DC of myeloid origin capture antigens in tissues, migrate to lymphoid organs and stimulate T cell responses. A subset of DC has been described which expresses lymphoid determinants and has potential regulatory functions. Conditional transformation of chicken bone marrow progenitors with v-relER, a v-rel estrogen receptor (ER) fusion gene, allows expansion of progenitors that can be induced to differentiate into DC in vitro. In this paper we describe that v-relER cells exhibit both myeloid and lymphoid surface markers, while B cell, T cell and NK (natural killer)-specific surface markers are absent. v-relER DC express, however, cytoplasmic CD3 protein and mRNA for CD8α and the lymphoid transcription factor GATA-3. These data suggest that v-relER DC might be related to the lymphoid subset of DC described in mammals.
UR - http://www.scopus.com/inward/record.url?scp=0033709122&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033709122&partnerID=8YFLogxK
M3 - Article
C2 - 11131155
AN - SCOPUS:0033709122
VL - 202
SP - 394
EP - 407
JO - Immunobiology
JF - Immunobiology
SN - 0171-2985
IS - 4
ER -