Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes

J. Madruga, Karoline Briegel, S. Diebold, G. Boehmelt, F. Vogel, M. Zenke

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The initiation of primary immune responses is the key function of specialized antigen presenting cells, the dendritic cells (DC). DC of myeloid origin capture antigens in tissues, migrate to lymphoid organs and stimulate T cell responses. A subset of DC has been described which expresses lymphoid determinants and has potential regulatory functions. Conditional transformation of chicken bone marrow progenitors with v-relER, a v-rel estrogen receptor (ER) fusion gene, allows expansion of progenitors that can be induced to differentiate into DC in vitro. In this paper we describe that v-relER cells exhibit both myeloid and lymphoid surface markers, while B cell, T cell and NK (natural killer)-specific surface markers are absent. v-relER DC express, however, cytoplasmic CD3 protein and mRNA for CD8α and the lymphoid transcription factor GATA-3. These data suggest that v-relER DC might be related to the lymphoid subset of DC described in mammals.

Original languageEnglish
Pages (from-to)394-407
Number of pages14
JournalImmunobiology
Volume202
Issue number4
StatePublished - Dec 7 2000
Externally publishedYes

Fingerprint

Oncogenes
Dendritic Cells
Genes
Transcription Factor 3
Natural Killer T-Cells
Gene Fusion
Antigen-Presenting Cells
Estrogen Receptors
Mammals
Chickens
B-Lymphocytes
Bone Marrow
T-Lymphocytes
Antigens
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Madruga, J., Briegel, K., Diebold, S., Boehmelt, G., Vogel, F., & Zenke, M. (2000). Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes. Immunobiology, 202(4), 394-407.

Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes. / Madruga, J.; Briegel, Karoline; Diebold, S.; Boehmelt, G.; Vogel, F.; Zenke, M.

In: Immunobiology, Vol. 202, No. 4, 07.12.2000, p. 394-407.

Research output: Contribution to journalArticle

Madruga, J, Briegel, K, Diebold, S, Boehmelt, G, Vogel, F & Zenke, M 2000, 'Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes', Immunobiology, vol. 202, no. 4, pp. 394-407.
Madruga J, Briegel K, Diebold S, Boehmelt G, Vogel F, Zenke M. Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes. Immunobiology. 2000 Dec 7;202(4):394-407.
Madruga, J. ; Briegel, Karoline ; Diebold, S. ; Boehmelt, G. ; Vogel, F. ; Zenke, M. / Dendritic cells conditionally transformed by v-relER oncogene express lymphoid marker genes. In: Immunobiology. 2000 ; Vol. 202, No. 4. pp. 394-407.
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