Abstract
Presentation of MHC class I antigens by professional antigen-presenting cells (APC) is an important pathway in priming cytotoxic T lymphocyte responses in vivo. This study sought to identify the nature of the professional APC responsible for indirect class I presentation by examining a special feature of professional APC, namely their ability to process exogenous forms of antigen for class I presentation. Incubation of highly purified bone marrow-derived precursor cells with chicken ovalbumin (OVA) led to the efficient presentation of the major class I-restricted OVA determinant by mature dendritic cells (DC), but not by macrophages (MΦ) derived from the precursor population. DC as well as macrophages were, however, able to mediate class II presentation of OVA, suggesting that macrophages were deficient in class I processing but not in capturing exogenous OVA. The majority of mature DC, i.e. over 80%, generated from the precursor cells pulsed with OVA, presented the class I OVA epitope. Upon maturation, class I presentation of OVA by DC was greatly reduced, suggesting that class I processing of exogenous antigen is modulated during DC maturation in a manner similar to class II antigen processing. This study shows that bone marrow-derived DC/MΦ progenitors capture exogenous antigen for class I presentation, and that cells of the DC lineage can be functionally distinguished from cells of the macrophage lineage based on their ability to process exogenous antigen for class I presentation.
| Original language | English |
|---|---|
| Pages (from-to) | 1923-1933 |
| Number of pages | 11 |
| Journal | European Journal of Immunology |
| Volume | 28 |
| Issue number | 6 |
| DOIs | |
| State | Published - Jun 1 1998 |
| Externally published | Yes |
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Keywords
- Antigen-presenting cell
- Cytotoxic T lymphocyte
- Dendritic cell
- Macrophage
- MHC class I
ASJC Scopus subject areas
- Immunology
Cite this
Dendritic cell/macrophage precursors capture exogenous antigen for MHC class I presentation by dendritic cells. / Mitchell, Duane A.; Nair, Smita K.; Gilboa, Eli.
In: European Journal of Immunology, Vol. 28, No. 6, 01.06.1998, p. 1923-1933.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dendritic cell/macrophage precursors capture exogenous antigen for MHC class I presentation by dendritic cells
AU - Mitchell, Duane A.
AU - Nair, Smita K.
AU - Gilboa, Eli
PY - 1998/6/1
Y1 - 1998/6/1
N2 - Presentation of MHC class I antigens by professional antigen-presenting cells (APC) is an important pathway in priming cytotoxic T lymphocyte responses in vivo. This study sought to identify the nature of the professional APC responsible for indirect class I presentation by examining a special feature of professional APC, namely their ability to process exogenous forms of antigen for class I presentation. Incubation of highly purified bone marrow-derived precursor cells with chicken ovalbumin (OVA) led to the efficient presentation of the major class I-restricted OVA determinant by mature dendritic cells (DC), but not by macrophages (MΦ) derived from the precursor population. DC as well as macrophages were, however, able to mediate class II presentation of OVA, suggesting that macrophages were deficient in class I processing but not in capturing exogenous OVA. The majority of mature DC, i.e. over 80%, generated from the precursor cells pulsed with OVA, presented the class I OVA epitope. Upon maturation, class I presentation of OVA by DC was greatly reduced, suggesting that class I processing of exogenous antigen is modulated during DC maturation in a manner similar to class II antigen processing. This study shows that bone marrow-derived DC/MΦ progenitors capture exogenous antigen for class I presentation, and that cells of the DC lineage can be functionally distinguished from cells of the macrophage lineage based on their ability to process exogenous antigen for class I presentation.
AB - Presentation of MHC class I antigens by professional antigen-presenting cells (APC) is an important pathway in priming cytotoxic T lymphocyte responses in vivo. This study sought to identify the nature of the professional APC responsible for indirect class I presentation by examining a special feature of professional APC, namely their ability to process exogenous forms of antigen for class I presentation. Incubation of highly purified bone marrow-derived precursor cells with chicken ovalbumin (OVA) led to the efficient presentation of the major class I-restricted OVA determinant by mature dendritic cells (DC), but not by macrophages (MΦ) derived from the precursor population. DC as well as macrophages were, however, able to mediate class II presentation of OVA, suggesting that macrophages were deficient in class I processing but not in capturing exogenous OVA. The majority of mature DC, i.e. over 80%, generated from the precursor cells pulsed with OVA, presented the class I OVA epitope. Upon maturation, class I presentation of OVA by DC was greatly reduced, suggesting that class I processing of exogenous antigen is modulated during DC maturation in a manner similar to class II antigen processing. This study shows that bone marrow-derived DC/MΦ progenitors capture exogenous antigen for class I presentation, and that cells of the DC lineage can be functionally distinguished from cells of the macrophage lineage based on their ability to process exogenous antigen for class I presentation.
KW - Antigen-presenting cell
KW - Cytotoxic T lymphocyte
KW - Dendritic cell
KW - Macrophage
KW - MHC class I
UR - http://www.scopus.com/inward/record.url?scp=0031867792&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031867792&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1521-4141(199806)28:06<1923::AID-IMMU1923>3.0.CO;2-9
DO - 10.1002/(SICI)1521-4141(199806)28:06<1923::AID-IMMU1923>3.0.CO;2-9
M3 - Article
C2 - 9645374
AN - SCOPUS:0031867792
VL - 28
SP - 1923
EP - 1933
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 6
ER -