Demonstration of bacterial biofilms in culture-negative silicone stent and jones tube

Kami Parsa, Christoph Schaudinn, Amita Gorur, Parish P. Sedghizadeh, Thomas Johnson, David Tse, John W. Costerton

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

PURPOSE:: To demonstrate the presence of bacterial biofilms on a dacryocystorhinostomy silicone stent and a Jones tube. METHODS:: One dacryocystorhinostomy silicone stent and one Jones tube were removed from 2 patients who presented with an infection of their respective nasolacrimal system. Cultures were obtained, and the implants were processed for scanning electron microscopy and confocal laser scanning microscopy, advanced microscopic methods that are applicable for detection of uncultivable biofilm organisms. RESULTS:: Routine bacterial cultures revealed no growth, but bacterial biofilms on outer and inner surfaces of both implants were confirmed by advanced microscopic techniques. CONCLUSIONS:: To the authors' knowledge, this is the first article that documents the presence of biofilms on a Crawford stent or a Jones tube on patients who presented with infections involving the nasolacrimal system. Although initial cultures revealed absence of any bacterial growth, confocal laser scanning microscopy and scanning electron microscopy documented bacterial colonization. Clinicians should consider the role of biofilms and the limitation of our standard culturing techniques while treating patients with device- or implant-related infections.

Original languageEnglish
Pages (from-to)426-430
Number of pages5
JournalOphthalmic Plastic and Reconstructive Surgery
Volume26
Issue number6
DOIs
StatePublished - Nov 1 2010

Fingerprint

Silicones
Biofilms
Stents
Dacryocystorhinostomy
Confocal Microscopy
Electron Scanning Microscopy
Infection
Growth
Equipment and Supplies

ASJC Scopus subject areas

  • Ophthalmology
  • Surgery

Cite this

Demonstration of bacterial biofilms in culture-negative silicone stent and jones tube. / Parsa, Kami; Schaudinn, Christoph; Gorur, Amita; Sedghizadeh, Parish P.; Johnson, Thomas; Tse, David; Costerton, John W.

In: Ophthalmic Plastic and Reconstructive Surgery, Vol. 26, No. 6, 01.11.2010, p. 426-430.

Research output: Contribution to journalArticle

Parsa, Kami ; Schaudinn, Christoph ; Gorur, Amita ; Sedghizadeh, Parish P. ; Johnson, Thomas ; Tse, David ; Costerton, John W. / Demonstration of bacterial biofilms in culture-negative silicone stent and jones tube. In: Ophthalmic Plastic and Reconstructive Surgery. 2010 ; Vol. 26, No. 6. pp. 426-430.
@article{a711daf3191141758dbe5366528f08ab,
title = "Demonstration of bacterial biofilms in culture-negative silicone stent and jones tube",
abstract = "PURPOSE:: To demonstrate the presence of bacterial biofilms on a dacryocystorhinostomy silicone stent and a Jones tube. METHODS:: One dacryocystorhinostomy silicone stent and one Jones tube were removed from 2 patients who presented with an infection of their respective nasolacrimal system. Cultures were obtained, and the implants were processed for scanning electron microscopy and confocal laser scanning microscopy, advanced microscopic methods that are applicable for detection of uncultivable biofilm organisms. RESULTS:: Routine bacterial cultures revealed no growth, but bacterial biofilms on outer and inner surfaces of both implants were confirmed by advanced microscopic techniques. CONCLUSIONS:: To the authors' knowledge, this is the first article that documents the presence of biofilms on a Crawford stent or a Jones tube on patients who presented with infections involving the nasolacrimal system. Although initial cultures revealed absence of any bacterial growth, confocal laser scanning microscopy and scanning electron microscopy documented bacterial colonization. Clinicians should consider the role of biofilms and the limitation of our standard culturing techniques while treating patients with device- or implant-related infections.",
author = "Kami Parsa and Christoph Schaudinn and Amita Gorur and Sedghizadeh, {Parish P.} and Thomas Johnson and David Tse and Costerton, {John W.}",
year = "2010",
month = "11",
day = "1",
doi = "10.1097/IOP.0b013e3181cff61f",
language = "English",
volume = "26",
pages = "426--430",
journal = "Ophthalmic Plastic and Reconstructive Surgery",
issn = "0740-9303",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Demonstration of bacterial biofilms in culture-negative silicone stent and jones tube

AU - Parsa, Kami

AU - Schaudinn, Christoph

AU - Gorur, Amita

AU - Sedghizadeh, Parish P.

AU - Johnson, Thomas

AU - Tse, David

AU - Costerton, John W.

PY - 2010/11/1

Y1 - 2010/11/1

N2 - PURPOSE:: To demonstrate the presence of bacterial biofilms on a dacryocystorhinostomy silicone stent and a Jones tube. METHODS:: One dacryocystorhinostomy silicone stent and one Jones tube were removed from 2 patients who presented with an infection of their respective nasolacrimal system. Cultures were obtained, and the implants were processed for scanning electron microscopy and confocal laser scanning microscopy, advanced microscopic methods that are applicable for detection of uncultivable biofilm organisms. RESULTS:: Routine bacterial cultures revealed no growth, but bacterial biofilms on outer and inner surfaces of both implants were confirmed by advanced microscopic techniques. CONCLUSIONS:: To the authors' knowledge, this is the first article that documents the presence of biofilms on a Crawford stent or a Jones tube on patients who presented with infections involving the nasolacrimal system. Although initial cultures revealed absence of any bacterial growth, confocal laser scanning microscopy and scanning electron microscopy documented bacterial colonization. Clinicians should consider the role of biofilms and the limitation of our standard culturing techniques while treating patients with device- or implant-related infections.

AB - PURPOSE:: To demonstrate the presence of bacterial biofilms on a dacryocystorhinostomy silicone stent and a Jones tube. METHODS:: One dacryocystorhinostomy silicone stent and one Jones tube were removed from 2 patients who presented with an infection of their respective nasolacrimal system. Cultures were obtained, and the implants were processed for scanning electron microscopy and confocal laser scanning microscopy, advanced microscopic methods that are applicable for detection of uncultivable biofilm organisms. RESULTS:: Routine bacterial cultures revealed no growth, but bacterial biofilms on outer and inner surfaces of both implants were confirmed by advanced microscopic techniques. CONCLUSIONS:: To the authors' knowledge, this is the first article that documents the presence of biofilms on a Crawford stent or a Jones tube on patients who presented with infections involving the nasolacrimal system. Although initial cultures revealed absence of any bacterial growth, confocal laser scanning microscopy and scanning electron microscopy documented bacterial colonization. Clinicians should consider the role of biofilms and the limitation of our standard culturing techniques while treating patients with device- or implant-related infections.

UR - http://www.scopus.com/inward/record.url?scp=78650510831&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650510831&partnerID=8YFLogxK

U2 - 10.1097/IOP.0b013e3181cff61f

DO - 10.1097/IOP.0b013e3181cff61f

M3 - Article

C2 - 20683273

AN - SCOPUS:78650510831

VL - 26

SP - 426

EP - 430

JO - Ophthalmic Plastic and Reconstructive Surgery

JF - Ophthalmic Plastic and Reconstructive Surgery

SN - 0740-9303

IS - 6

ER -