Abstract
Identification of regulatory elements in the human renin gene promoter has been hindered by the lack of suitable renin-expressing cell lines. In this paper, the SV40 viral enhancer is coupled to a human renin promoter/chloramphenicol acetyltransferase (CAT) reporter gene construct, to determine whether promoter regulatory elements can be identified in the context of enhanced transcription in the normally non-renin-expressing HeLa cell line. The present results indicate that the SV40 enhancer can overcome tissue-specificity of the human renin promoter, and confer correctly initiated transcriptional activity to HeLa cells. Analysis of a series of 5'-end deletions of the human renin promoter linked to the CAT gene and SV40 enhancer identified negative regulatory elements between positions-275 and -225, and between-142 and -102 of the human renin promoter, as well as a positive regulatory element between -225 and -142. Thus, studies of renin promoter regulatory elements need not be limited to renin-expressing cells, but can be performed in non-renin-expressing cells with the addition of an enhancer. This strategy can be generally applicable to the study of tissue-specific gene regulation in cases where there are no specific cell lines available.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-91 |
| Number of pages | 13 |
| Journal | The Bulletin of Tokyo Medical and Dental University |
| Volume | 40 |
| Issue number | 2 |
| State | Published - Jun 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Deletional analysis of the human renin promoter : transcriptional activation by the SV40 enhancer enables identification of promoter regulatory elements in non-renin-expressing cells. / Kasahara, Noriyuki.
In: The Bulletin of Tokyo Medical and Dental University, Vol. 40, No. 2, 06.1993, p. 79-91.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Deletional analysis of the human renin promoter
T2 - transcriptional activation by the SV40 enhancer enables identification of promoter regulatory elements in non-renin-expressing cells.
AU - Kasahara, Noriyuki
PY - 1993/6
Y1 - 1993/6
N2 - Identification of regulatory elements in the human renin gene promoter has been hindered by the lack of suitable renin-expressing cell lines. In this paper, the SV40 viral enhancer is coupled to a human renin promoter/chloramphenicol acetyltransferase (CAT) reporter gene construct, to determine whether promoter regulatory elements can be identified in the context of enhanced transcription in the normally non-renin-expressing HeLa cell line. The present results indicate that the SV40 enhancer can overcome tissue-specificity of the human renin promoter, and confer correctly initiated transcriptional activity to HeLa cells. Analysis of a series of 5'-end deletions of the human renin promoter linked to the CAT gene and SV40 enhancer identified negative regulatory elements between positions-275 and -225, and between-142 and -102 of the human renin promoter, as well as a positive regulatory element between -225 and -142. Thus, studies of renin promoter regulatory elements need not be limited to renin-expressing cells, but can be performed in non-renin-expressing cells with the addition of an enhancer. This strategy can be generally applicable to the study of tissue-specific gene regulation in cases where there are no specific cell lines available.
AB - Identification of regulatory elements in the human renin gene promoter has been hindered by the lack of suitable renin-expressing cell lines. In this paper, the SV40 viral enhancer is coupled to a human renin promoter/chloramphenicol acetyltransferase (CAT) reporter gene construct, to determine whether promoter regulatory elements can be identified in the context of enhanced transcription in the normally non-renin-expressing HeLa cell line. The present results indicate that the SV40 enhancer can overcome tissue-specificity of the human renin promoter, and confer correctly initiated transcriptional activity to HeLa cells. Analysis of a series of 5'-end deletions of the human renin promoter linked to the CAT gene and SV40 enhancer identified negative regulatory elements between positions-275 and -225, and between-142 and -102 of the human renin promoter, as well as a positive regulatory element between -225 and -142. Thus, studies of renin promoter regulatory elements need not be limited to renin-expressing cells, but can be performed in non-renin-expressing cells with the addition of an enhancer. This strategy can be generally applicable to the study of tissue-specific gene regulation in cases where there are no specific cell lines available.
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UR - http://www.scopus.com/inward/citedby.url?scp=0027615856&partnerID=8YFLogxK
M3 - Article
C2 - 8390924
AN - SCOPUS:0027615856
VL - 40
SP - 79
EP - 91
JO - Journal of Medical and Dental Sciences
JF - Journal of Medical and Dental Sciences
SN - 1342-8810
IS - 2
ER -