Deletion of the epidermis derived laminin γ1 chain leads to defects in the regulation of late hair morphogenesis

Anja Fleger-Weckmann, Yasemin Üstün, Jennifer Kloepper, Ralf Paus, Wilhelm Bloch, Zu Lin Chen, Jeannine Wegner, Lydia Sorokin, Lutz Langbein, Beate Eckes, Paola Zigrino, Thomas Krieg, Roswitha Nischt

Research output: Contribution to journalArticle

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Abstract

Laminins are the most abundant non-collagenous basement membrane (BM) components, composed of an α, β and γ chain. The laminin γ1 chain, encoded by LAMC1, is the most abundant γ chain. The main laminin isoforms in the dermo-epidermal junction (DEJ) are laminin-332, laminin-511 and laminin-211, the latter being restricted to the lower part of hair follicles (HFs). Complete deletion of LAMC1 results in lethality around embryonic day 5.5. To study the function of laminin γ1 containing isoforms in skin development and maturation after birth, we generated mice lacking LAMC1 expression in basal keratinocytes (LAMC1EKO) using the keratin 14 (K14) Cre/loxP system. This deletion resulted in loss of keratinocyte derived laminin-511 and in deposition of fibroblast derived laminin-211 throughout the whole DEJ. The DEJ in areas between hemidesmosomes was thickened, whereas hemidesmosome morphology was normal. Most strikingly, LAMC1EKO mice showed delayed HF morphogenesis accompanied by reduced proliferation of hair matrix cells and impaired differentiation of hair shafts (HS). However, this deletion did not interfere with early HF development, since placode numbers and embryonic hair germ formation were not affected. Microarray analysis of skin revealed down regulation of mainly different hair keratins. This is due to reduced expression of transcription factors such as HoxC13, FoxN1, FoxQ1 and Msx2, known to regulate expression of hair keratins. While the role of laminin-511 in signaling during early hair germ formation and elongation phase has been described, we here demonstrate that epidermal laminin-511 is also a key regulator for later hair development and HS differentiation.

Original languageEnglish (US)
Pages (from-to)42-56
Number of pages15
JournalMatrix Biology
Volume56
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

Fingerprint

Morphogenesis
Epidermis
Hair
Laminin
Hair Follicle
Hair-Specific Keratins
Keratinocytes
Hemidesmosomes
Protein Isoforms
Keratin-14
Skin
Microarray Analysis
Basement Membrane
laminin 1
Cell Differentiation
Transcription Factors
Down-Regulation
Fibroblasts
Parturition
laminin 10

Keywords

  • Basement membrane
  • Hair follicle
  • Laminin-211
  • Laminin-511
  • Skin

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Deletion of the epidermis derived laminin γ1 chain leads to defects in the regulation of late hair morphogenesis. / Fleger-Weckmann, Anja; Üstün, Yasemin; Kloepper, Jennifer; Paus, Ralf; Bloch, Wilhelm; Chen, Zu Lin; Wegner, Jeannine; Sorokin, Lydia; Langbein, Lutz; Eckes, Beate; Zigrino, Paola; Krieg, Thomas; Nischt, Roswitha.

In: Matrix Biology, Vol. 56, 01.12.2016, p. 42-56.

Research output: Contribution to journalArticle

Fleger-Weckmann, A, Üstün, Y, Kloepper, J, Paus, R, Bloch, W, Chen, ZL, Wegner, J, Sorokin, L, Langbein, L, Eckes, B, Zigrino, P, Krieg, T & Nischt, R 2016, 'Deletion of the epidermis derived laminin γ1 chain leads to defects in the regulation of late hair morphogenesis', Matrix Biology, vol. 56, pp. 42-56. https://doi.org/10.1016/j.matbio.2016.05.002
Fleger-Weckmann, Anja ; Üstün, Yasemin ; Kloepper, Jennifer ; Paus, Ralf ; Bloch, Wilhelm ; Chen, Zu Lin ; Wegner, Jeannine ; Sorokin, Lydia ; Langbein, Lutz ; Eckes, Beate ; Zigrino, Paola ; Krieg, Thomas ; Nischt, Roswitha. / Deletion of the epidermis derived laminin γ1 chain leads to defects in the regulation of late hair morphogenesis. In: Matrix Biology. 2016 ; Vol. 56. pp. 42-56.
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abstract = "Laminins are the most abundant non-collagenous basement membrane (BM) components, composed of an α, β and γ chain. The laminin γ1 chain, encoded by LAMC1, is the most abundant γ chain. The main laminin isoforms in the dermo-epidermal junction (DEJ) are laminin-332, laminin-511 and laminin-211, the latter being restricted to the lower part of hair follicles (HFs). Complete deletion of LAMC1 results in lethality around embryonic day 5.5. To study the function of laminin γ1 containing isoforms in skin development and maturation after birth, we generated mice lacking LAMC1 expression in basal keratinocytes (LAMC1EKO) using the keratin 14 (K14) Cre/loxP system. This deletion resulted in loss of keratinocyte derived laminin-511 and in deposition of fibroblast derived laminin-211 throughout the whole DEJ. The DEJ in areas between hemidesmosomes was thickened, whereas hemidesmosome morphology was normal. Most strikingly, LAMC1EKO mice showed delayed HF morphogenesis accompanied by reduced proliferation of hair matrix cells and impaired differentiation of hair shafts (HS). However, this deletion did not interfere with early HF development, since placode numbers and embryonic hair germ formation were not affected. Microarray analysis of skin revealed down regulation of mainly different hair keratins. This is due to reduced expression of transcription factors such as HoxC13, FoxN1, FoxQ1 and Msx2, known to regulate expression of hair keratins. While the role of laminin-511 in signaling during early hair germ formation and elongation phase has been described, we here demonstrate that epidermal laminin-511 is also a key regulator for later hair development and HS differentiation.",
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AU - Bloch, Wilhelm

AU - Chen, Zu Lin

AU - Wegner, Jeannine

AU - Sorokin, Lydia

AU - Langbein, Lutz

AU - Eckes, Beate

AU - Zigrino, Paola

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