Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation

S. C S Murthy, J. J. Trimble, Ronald Charles Desrosiers

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Analysis of a 5,549-base-pair sequence at the left end of herpesvirus saimiri unique (L-) DNA revealed two open reading frames and genes for five small nuclear U RNAs (herpesvirus saimiri U RNAs). Replication-competent deletion mutants were constructed in order to assess the importance of these genetic features for transformation by this oncogenic herpesvirus. Although not required for replication, one of the open reading frames was found to be required for immortalization of marmoset T lymphocytes into continuously growing cell lines. The protein predicted by this reading frame (STP; saimiri transformation-associated protein) has a highly hydrophobic stretch of 26 amino acids sufficient for a membrane-spanning domain near its carboxy terminus; this domain is immediately preceded by a sequence appropriate for formation of a metal-binding domain (His X2 His X6 Cys X2 Cys, where Xs are other amino acids). One of two poly(A)+ RNAs that could encode STP is bicistronic, while the other has a long 5' untranslated region (~ 1.5 kilobases). Although some analogies can be drawn between STP and LMP (lymphocyte membrane protein) of Epstein-Barr virus, STP is not related in sequence to LMP.

Original languageEnglish (US)
Pages (from-to)3307-3314
Number of pages8
JournalJournal of Virology
Volume63
Issue number8
StatePublished - 1989
Externally publishedYes

Fingerprint

Saimiriine herpesvirus 2
Saimiriine Herpesvirus 2
membrane proteins
Open Reading Frames
open reading frames
Membrane Proteins
lymphocytes
RNA
Lymphocytes
Small Nuclear RNA
Genetic Transformation
Amino Acids
Human herpesvirus 4
Callithrix
Saimiri
Reading Frames
mutants
amino acids
5' Untranslated Regions
Herpesviridae

ASJC Scopus subject areas

  • Immunology

Cite this

Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation. / Murthy, S. C S; Trimble, J. J.; Desrosiers, Ronald Charles.

In: Journal of Virology, Vol. 63, No. 8, 1989, p. 3307-3314.

Research output: Contribution to journalArticle

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