Deleted in colorectal cancer is a putative conditional tumor-suppressor gene inactivated by promoter hypermethylation in head and neck squamous cell carcinoma

André Lopes Carvalho, Alice Chuang, Wei Wen Jiang, Juna Lee, Shahnaz Begum, Luana Poeta, Ming Zhao, Carmen Jerónimo, Rui Henrique, Chetan Nayak, Hannah L. Park, Mariana R O Brait, Chunyan Liu, Shaoyu Zhou, Wayne Koch, Vito Michele Fazio, Edward Ratovitski, Barry Trink, William Westra, David Sidransky & 2 others Chul So Moon, Joseph A. Califano

Research output: Contribution to journalArticle

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Abstract

Deleted in colorectal cancer (DCC) is a candidate tumor-suppressor gene located at chromosome 18q21. However, DCC gene was found to have few somatic mutations and the heterozygous mice (DCC+/-) showed a similar frequency of tumor formation compared with the wild-type mice (DCC +/+). Recently, DCC came back to the spotlight as a better understating of its function and relationship with its ligand (netrin-1) had shown that DCC may act as a conditional tumor-suppressor gene. We evaluated hypermethylation as a mechanism for DCC inactivation in head and neck squamous cell carcinoma (HNSCC). DCC promoter region hypermethylation was found in 75% of primary HNSCC. There was a significant correlation between DCC promoter region hypermethylation and DCC expression (assessed by immunohistochemistry; P = 0.021). DCC nonexpressing HNSCC cell lines JHU-O12 and JHU-O19 with baseline hypermethylation of the DCC promoter were treated with 5-aza-2′- deoxycytidine (a demethylating agent) and reexpression of DCC was noted. Transfection of DCC into DCC-negative HNSCC cell lines resulted in complete abrogation of growth in all cell lines, whereas additional cotransfection of netrin-1 resulted in rescue of DCC-mediated growth inhibition. These results suggest that DCC is a putative conditional tumor-suppressor gene that is epigenetically inactivated by promoter hypermethylation in a majority of HNSCC.

Original languageEnglish
Pages (from-to)9401-9407
Number of pages7
JournalCancer Research
Volume66
Issue number19
DOIs
StatePublished - Oct 1 2006
Externally publishedYes

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Tumor Suppressor Genes
Colorectal Neoplasms
Carcinoma, squamous cell of head and neck
decitabine
Head and Neck Neoplasms
Genetic Promoter Regions
Cell Line
Neoplasm Genes
Growth
Transfection

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Deleted in colorectal cancer is a putative conditional tumor-suppressor gene inactivated by promoter hypermethylation in head and neck squamous cell carcinoma. / Carvalho, André Lopes; Chuang, Alice; Jiang, Wei Wen; Lee, Juna; Begum, Shahnaz; Poeta, Luana; Zhao, Ming; Jerónimo, Carmen; Henrique, Rui; Nayak, Chetan; Park, Hannah L.; Brait, Mariana R O; Liu, Chunyan; Zhou, Shaoyu; Koch, Wayne; Fazio, Vito Michele; Ratovitski, Edward; Trink, Barry; Westra, William; Sidransky, David; Moon, Chul So; Califano, Joseph A.

In: Cancer Research, Vol. 66, No. 19, 01.10.2006, p. 9401-9407.

Research output: Contribution to journalArticle

Carvalho, AL, Chuang, A, Jiang, WW, Lee, J, Begum, S, Poeta, L, Zhao, M, Jerónimo, C, Henrique, R, Nayak, C, Park, HL, Brait, MRO, Liu, C, Zhou, S, Koch, W, Fazio, VM, Ratovitski, E, Trink, B, Westra, W, Sidransky, D, Moon, CS & Califano, JA 2006, 'Deleted in colorectal cancer is a putative conditional tumor-suppressor gene inactivated by promoter hypermethylation in head and neck squamous cell carcinoma', Cancer Research, vol. 66, no. 19, pp. 9401-9407. https://doi.org/10.1158/0008-5472.CAN-06-1073
Carvalho, André Lopes ; Chuang, Alice ; Jiang, Wei Wen ; Lee, Juna ; Begum, Shahnaz ; Poeta, Luana ; Zhao, Ming ; Jerónimo, Carmen ; Henrique, Rui ; Nayak, Chetan ; Park, Hannah L. ; Brait, Mariana R O ; Liu, Chunyan ; Zhou, Shaoyu ; Koch, Wayne ; Fazio, Vito Michele ; Ratovitski, Edward ; Trink, Barry ; Westra, William ; Sidransky, David ; Moon, Chul So ; Califano, Joseph A. / Deleted in colorectal cancer is a putative conditional tumor-suppressor gene inactivated by promoter hypermethylation in head and neck squamous cell carcinoma. In: Cancer Research. 2006 ; Vol. 66, No. 19. pp. 9401-9407.
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title = "Deleted in colorectal cancer is a putative conditional tumor-suppressor gene inactivated by promoter hypermethylation in head and neck squamous cell carcinoma",
abstract = "Deleted in colorectal cancer (DCC) is a candidate tumor-suppressor gene located at chromosome 18q21. However, DCC gene was found to have few somatic mutations and the heterozygous mice (DCC+/-) showed a similar frequency of tumor formation compared with the wild-type mice (DCC +/+). Recently, DCC came back to the spotlight as a better understating of its function and relationship with its ligand (netrin-1) had shown that DCC may act as a conditional tumor-suppressor gene. We evaluated hypermethylation as a mechanism for DCC inactivation in head and neck squamous cell carcinoma (HNSCC). DCC promoter region hypermethylation was found in 75{\%} of primary HNSCC. There was a significant correlation between DCC promoter region hypermethylation and DCC expression (assessed by immunohistochemistry; P = 0.021). DCC nonexpressing HNSCC cell lines JHU-O12 and JHU-O19 with baseline hypermethylation of the DCC promoter were treated with 5-aza-2′- deoxycytidine (a demethylating agent) and reexpression of DCC was noted. Transfection of DCC into DCC-negative HNSCC cell lines resulted in complete abrogation of growth in all cell lines, whereas additional cotransfection of netrin-1 resulted in rescue of DCC-mediated growth inhibition. These results suggest that DCC is a putative conditional tumor-suppressor gene that is epigenetically inactivated by promoter hypermethylation in a majority of HNSCC.",
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T1 - Deleted in colorectal cancer is a putative conditional tumor-suppressor gene inactivated by promoter hypermethylation in head and neck squamous cell carcinoma

AU - Carvalho, André Lopes

AU - Chuang, Alice

AU - Jiang, Wei Wen

AU - Lee, Juna

AU - Begum, Shahnaz

AU - Poeta, Luana

AU - Zhao, Ming

AU - Jerónimo, Carmen

AU - Henrique, Rui

AU - Nayak, Chetan

AU - Park, Hannah L.

AU - Brait, Mariana R O

AU - Liu, Chunyan

AU - Zhou, Shaoyu

AU - Koch, Wayne

AU - Fazio, Vito Michele

AU - Ratovitski, Edward

AU - Trink, Barry

AU - Westra, William

AU - Sidransky, David

AU - Moon, Chul So

AU - Califano, Joseph A.

PY - 2006/10/1

Y1 - 2006/10/1

N2 - Deleted in colorectal cancer (DCC) is a candidate tumor-suppressor gene located at chromosome 18q21. However, DCC gene was found to have few somatic mutations and the heterozygous mice (DCC+/-) showed a similar frequency of tumor formation compared with the wild-type mice (DCC +/+). Recently, DCC came back to the spotlight as a better understating of its function and relationship with its ligand (netrin-1) had shown that DCC may act as a conditional tumor-suppressor gene. We evaluated hypermethylation as a mechanism for DCC inactivation in head and neck squamous cell carcinoma (HNSCC). DCC promoter region hypermethylation was found in 75% of primary HNSCC. There was a significant correlation between DCC promoter region hypermethylation and DCC expression (assessed by immunohistochemistry; P = 0.021). DCC nonexpressing HNSCC cell lines JHU-O12 and JHU-O19 with baseline hypermethylation of the DCC promoter were treated with 5-aza-2′- deoxycytidine (a demethylating agent) and reexpression of DCC was noted. Transfection of DCC into DCC-negative HNSCC cell lines resulted in complete abrogation of growth in all cell lines, whereas additional cotransfection of netrin-1 resulted in rescue of DCC-mediated growth inhibition. These results suggest that DCC is a putative conditional tumor-suppressor gene that is epigenetically inactivated by promoter hypermethylation in a majority of HNSCC.

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