Delayed disappearance of 14C-labeled-Pro-Leu-Gly-NH2 from the blood of hypophysectomized rats

A. J. Kastin, Cheryl Nissen, T. W. Redding, R. M.G. Nair, A. V. Schally

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The disappearance of radioactivity after the injection of 14C-labeled-Pro-Leu-Gly-NH2, an MSH releaseinhibiting factor (MIF-I), was measured in rats which had been subjected to various procedures. Delayed disappearance of the radioactivity to a small but statistically highly significant extent was found in hypophysectomized rats compared to rats with an intact pituitary. Pinealectomy and adrenalectomy did not change the half-time disappearance. Dehydration, hypothalamic destruction, or administration of excess saline, MSH, and large amounts of unlabeled MIF-I were all used as control procedures. These failed to demonstrate the mechanism by which the prolonged disappearance occurred, but did raise other questions. The half-time disappearance of tritiated inulin was not significantly different in hypophysectomized or intact rats. The results indicate that the absence of the pituitary gland prolongs the half-time disappearance of radioactivity from the blood of rats injected with labeled MIF-I.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalNeuroendocrinology
Volume16
Issue number1
DOIs
StatePublished - Jan 1 1974
Externally publishedYes

Keywords

  • C-MIF
  • Hypophysectomized rats
  • Radioactivity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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