Delayed disappearance of 14C-labeled-Pro-Leu-Gly-NH2 from the blood of hypophysectomized rats

A. J. Kastin, Cheryl Nissen, T. W. Redding, R. M G Nair, Andrew V Schally

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The disappearance of radioactivity after the injection of 14C-labeled-Pro-Leu-Gly-NH2, an MSH releaseinhibiting factor (MIF-I), was measured in rats which had been subjected to various procedures. Delayed disappearance of the radioactivity to a small but statistically highly significant extent was found in hypophysectomized rats compared to rats with an intact pituitary. Pinealectomy and adrenalectomy did not change the half-time disappearance. Dehydration, hypothalamic destruction, or administration of excess saline, MSH, and large amounts of unlabeled MIF-I were all used as control procedures. These failed to demonstrate the mechanism by which the prolonged disappearance occurred, but did raise other questions. The half-time disappearance of tritiated inulin was not significantly different in hypophysectomized or intact rats. The results indicate that the absence of the pituitary gland prolongs the half-time disappearance of radioactivity from the blood of rats injected with labeled MIF-I.

Original languageEnglish (US)
Pages (from-to)36-42
Number of pages7
JournalNeuroendocrinology
Volume16
Issue number1
DOIs
StatePublished - 1974
Externally publishedYes

Fingerprint

MSH Release-Inhibiting Hormone
Radioactivity
Melanocyte-Stimulating Hormones
Inulin
Adrenalectomy
Pituitary Gland
Dehydration
Injections

Keywords

  • <sup>14</sup>C-MIF
  • Hypophysectomized rats
  • Radioactivity

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Delayed disappearance of 14C-labeled-Pro-Leu-Gly-NH2 from the blood of hypophysectomized rats. / Kastin, A. J.; Nissen, Cheryl; Redding, T. W.; Nair, R. M G; Schally, Andrew V.

In: Neuroendocrinology, Vol. 16, No. 1, 1974, p. 36-42.

Research output: Contribution to journalArticle

Kastin, A. J. ; Nissen, Cheryl ; Redding, T. W. ; Nair, R. M G ; Schally, Andrew V. / Delayed disappearance of 14C-labeled-Pro-Leu-Gly-NH2 from the blood of hypophysectomized rats. In: Neuroendocrinology. 1974 ; Vol. 16, No. 1. pp. 36-42.
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