Abstract
The disappearance of radioactivity after the injection of 14C-labeled-Pro-Leu-Gly-NH2, an MSH releaseinhibiting factor (MIF-I), was measured in rats which had been subjected to various procedures. Delayed disappearance of the radioactivity to a small but statistically highly significant extent was found in hypophysectomized rats compared to rats with an intact pituitary. Pinealectomy and adrenalectomy did not change the half-time disappearance. Dehydration, hypothalamic destruction, or administration of excess saline, MSH, and large amounts of unlabeled MIF-I were all used as control procedures. These failed to demonstrate the mechanism by which the prolonged disappearance occurred, but did raise other questions. The half-time disappearance of tritiated inulin was not significantly different in hypophysectomized or intact rats. The results indicate that the absence of the pituitary gland prolongs the half-time disappearance of radioactivity from the blood of rats injected with labeled MIF-I.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 36-42 |
| Number of pages | 7 |
| Journal | Neuroendocrinology |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1974 |
| Externally published | Yes |
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Keywords
- <sup>14</sup>C-MIF
- Hypophysectomized rats
- Radioactivity
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
- Cellular and Molecular Neuroscience
- Endocrine and Autonomic Systems
- Neuroscience(all)
Cite this
Delayed disappearance of 14C-labeled-Pro-Leu-Gly-NH2 from the blood of hypophysectomized rats. / Kastin, A. J.; Nissen, Cheryl; Redding, T. W.; Nair, R. M G; Schally, Andrew V.
In: Neuroendocrinology, Vol. 16, No. 1, 1974, p. 36-42.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Delayed disappearance of 14C-labeled-Pro-Leu-Gly-NH2 from the blood of hypophysectomized rats
AU - Kastin, A. J.
AU - Nissen, Cheryl
AU - Redding, T. W.
AU - Nair, R. M G
AU - Schally, Andrew V
PY - 1974
Y1 - 1974
N2 - The disappearance of radioactivity after the injection of 14C-labeled-Pro-Leu-Gly-NH2, an MSH releaseinhibiting factor (MIF-I), was measured in rats which had been subjected to various procedures. Delayed disappearance of the radioactivity to a small but statistically highly significant extent was found in hypophysectomized rats compared to rats with an intact pituitary. Pinealectomy and adrenalectomy did not change the half-time disappearance. Dehydration, hypothalamic destruction, or administration of excess saline, MSH, and large amounts of unlabeled MIF-I were all used as control procedures. These failed to demonstrate the mechanism by which the prolonged disappearance occurred, but did raise other questions. The half-time disappearance of tritiated inulin was not significantly different in hypophysectomized or intact rats. The results indicate that the absence of the pituitary gland prolongs the half-time disappearance of radioactivity from the blood of rats injected with labeled MIF-I.
AB - The disappearance of radioactivity after the injection of 14C-labeled-Pro-Leu-Gly-NH2, an MSH releaseinhibiting factor (MIF-I), was measured in rats which had been subjected to various procedures. Delayed disappearance of the radioactivity to a small but statistically highly significant extent was found in hypophysectomized rats compared to rats with an intact pituitary. Pinealectomy and adrenalectomy did not change the half-time disappearance. Dehydration, hypothalamic destruction, or administration of excess saline, MSH, and large amounts of unlabeled MIF-I were all used as control procedures. These failed to demonstrate the mechanism by which the prolonged disappearance occurred, but did raise other questions. The half-time disappearance of tritiated inulin was not significantly different in hypophysectomized or intact rats. The results indicate that the absence of the pituitary gland prolongs the half-time disappearance of radioactivity from the blood of rats injected with labeled MIF-I.
KW - <sup>14</sup>C-MIF
KW - Hypophysectomized rats
KW - Radioactivity
UR - http://www.scopus.com/inward/record.url?scp=0016140591&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0016140591&partnerID=8YFLogxK
U2 - 10.1159/000122550
DO - 10.1159/000122550
M3 - Article
C2 - 4614100
AN - SCOPUS:0016140591
VL - 16
SP - 36
EP - 42
JO - Neuroendocrinology
JF - Neuroendocrinology
SN - 0028-3835
IS - 1
ER -