Degradation of myosin light chain in isolated rat hearts subjected to ischemia-reperfusion injury: A new intracellular target for matrix metalloproteinase-2

Grzegorz Sawicki, Hernando Leon, Jolanta Sawicka, Meltem Sariahmetoglu, Costas J. Schulze, Paul G. Scott, Danuta Szczesna-Cordary, Richard Schulz

Research output: Contribution to journalArticle

202 Citations (Scopus)

Abstract

Background - Matrix metalloproteinase-2 (MMP-2) contributes to cardiac dysfunction resulting from ischemia-reperfusion (I/R) injury. MMP-2 not only remodels the extracellular matrix but also acts intracellularly in I/R by degrading troponin I. Whether other intracellular targets exist for MMP-2 during I/R is unknown. Methods and Results - Isolated rat hearts were subjected to 20 minutes of ischemia and 30 minutes of reperfusion. The impaired recovery of mechanical function of the heart was attenuated by the MMP inhibitors o-phenanthroline or doxycycline. Quantitative 2D electrophoresis of homogenates of aerobically perfused hearts (control) or those subjected to I/R injury (in the presence or absence of MMP inhibitors) showed 3 low-molecular-weight proteins with levels that were significantly increased upon I/R injury and normalized to control levels by MMP inhibitors. Mass spectrometry analysis identified all 3 proteins as fragments of myosin light chain 1, which possesses theoretical cleavage recognition sequences for MMP-2 and is rapidly degraded by it in vitro. The association of MMP-2 with the thick myofilament in fractions prepared from I/R hearts was observed with immunogold electron microscopy, gelatin zymography for MMP-2 activity, and immunoprecipitation. MMP-2 was found to cleave myosin light chain 1 between tyrosine 189 and glutamine 190 at the C terminus. Conclusions - Our results demonstrate that myosin light chain 1 is another novel substrate for MMP-2 in the cardiomyocyte and that its degradation may contribute to contractile dysfunction resulting from I/R injury to the heart.

Original languageEnglish
Pages (from-to)544-552
Number of pages9
JournalCirculation
Volume112
Issue number4
DOIs
StatePublished - Jul 26 2005

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Myosin Light Chains
Matrix Metalloproteinase 2
Reperfusion Injury
Reperfusion
Matrix Metalloproteinase Inhibitors
Ischemia
Troponin I
Myofibrils
Doxycycline
Recovery of Function
Gelatin
Glutamine
Immunoprecipitation
Cardiac Myocytes
Extracellular Matrix
Tyrosine
Electrophoresis
Mass Spectrometry
Electron Microscopy
Proteins

Keywords

  • Metalloproteinases
  • Myocardial stunning
  • Myosin
  • Proteins
  • Reperfusion

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Degradation of myosin light chain in isolated rat hearts subjected to ischemia-reperfusion injury : A new intracellular target for matrix metalloproteinase-2. / Sawicki, Grzegorz; Leon, Hernando; Sawicka, Jolanta; Sariahmetoglu, Meltem; Schulze, Costas J.; Scott, Paul G.; Szczesna-Cordary, Danuta; Schulz, Richard.

In: Circulation, Vol. 112, No. 4, 26.07.2005, p. 544-552.

Research output: Contribution to journalArticle

Sawicki, Grzegorz ; Leon, Hernando ; Sawicka, Jolanta ; Sariahmetoglu, Meltem ; Schulze, Costas J. ; Scott, Paul G. ; Szczesna-Cordary, Danuta ; Schulz, Richard. / Degradation of myosin light chain in isolated rat hearts subjected to ischemia-reperfusion injury : A new intracellular target for matrix metalloproteinase-2. In: Circulation. 2005 ; Vol. 112, No. 4. pp. 544-552.
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AU - Leon, Hernando

AU - Sawicka, Jolanta

AU - Sariahmetoglu, Meltem

AU - Schulze, Costas J.

AU - Scott, Paul G.

AU - Szczesna-Cordary, Danuta

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N2 - Background - Matrix metalloproteinase-2 (MMP-2) contributes to cardiac dysfunction resulting from ischemia-reperfusion (I/R) injury. MMP-2 not only remodels the extracellular matrix but also acts intracellularly in I/R by degrading troponin I. Whether other intracellular targets exist for MMP-2 during I/R is unknown. Methods and Results - Isolated rat hearts were subjected to 20 minutes of ischemia and 30 minutes of reperfusion. The impaired recovery of mechanical function of the heart was attenuated by the MMP inhibitors o-phenanthroline or doxycycline. Quantitative 2D electrophoresis of homogenates of aerobically perfused hearts (control) or those subjected to I/R injury (in the presence or absence of MMP inhibitors) showed 3 low-molecular-weight proteins with levels that were significantly increased upon I/R injury and normalized to control levels by MMP inhibitors. Mass spectrometry analysis identified all 3 proteins as fragments of myosin light chain 1, which possesses theoretical cleavage recognition sequences for MMP-2 and is rapidly degraded by it in vitro. The association of MMP-2 with the thick myofilament in fractions prepared from I/R hearts was observed with immunogold electron microscopy, gelatin zymography for MMP-2 activity, and immunoprecipitation. MMP-2 was found to cleave myosin light chain 1 between tyrosine 189 and glutamine 190 at the C terminus. Conclusions - Our results demonstrate that myosin light chain 1 is another novel substrate for MMP-2 in the cardiomyocyte and that its degradation may contribute to contractile dysfunction resulting from I/R injury to the heart.

AB - Background - Matrix metalloproteinase-2 (MMP-2) contributes to cardiac dysfunction resulting from ischemia-reperfusion (I/R) injury. MMP-2 not only remodels the extracellular matrix but also acts intracellularly in I/R by degrading troponin I. Whether other intracellular targets exist for MMP-2 during I/R is unknown. Methods and Results - Isolated rat hearts were subjected to 20 minutes of ischemia and 30 minutes of reperfusion. The impaired recovery of mechanical function of the heart was attenuated by the MMP inhibitors o-phenanthroline or doxycycline. Quantitative 2D electrophoresis of homogenates of aerobically perfused hearts (control) or those subjected to I/R injury (in the presence or absence of MMP inhibitors) showed 3 low-molecular-weight proteins with levels that were significantly increased upon I/R injury and normalized to control levels by MMP inhibitors. Mass spectrometry analysis identified all 3 proteins as fragments of myosin light chain 1, which possesses theoretical cleavage recognition sequences for MMP-2 and is rapidly degraded by it in vitro. The association of MMP-2 with the thick myofilament in fractions prepared from I/R hearts was observed with immunogold electron microscopy, gelatin zymography for MMP-2 activity, and immunoprecipitation. MMP-2 was found to cleave myosin light chain 1 between tyrosine 189 and glutamine 190 at the C terminus. Conclusions - Our results demonstrate that myosin light chain 1 is another novel substrate for MMP-2 in the cardiomyocyte and that its degradation may contribute to contractile dysfunction resulting from I/R injury to the heart.

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KW - Reperfusion

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