Degradation of basement membranes by human matrix metalloproteinase 3 (stromelysin)

P. A. Bejarano, M. E. Noelken, K. Suzuki, B. G. Hudson, H. Nagase

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Connective tissue cells synthesize and secrete a group of matrix metalloproteinases (MMPs), all of which are capable of degrading the extracellular-matrix components. One of them, MMP-3 (stromelysin) has been shown to degrade purified basement-membrane components, collagen IV and laminin [Okada, Y., Nagase, H. & Harris, E.D., Jr. (1986) J. Biol. Chem. 261, 14245-14255]. Here we report that MMP-3 degrades collagen IV and laminin in intact basement membranes from bovine glomeruli (GBM) and bovine anterior-lens capsules (LBM). Degradation products were analysed by SDS/polyacrylamide-gel electrophoresis to determine the number and sizes of polypeptide fragments. Immunoblotting techniques were used to identify the origins of the fragments, i.e. collagen IV or laminin. The fragments of collagen IV were further mapped using specific antibodies that recognize the N-terminal (7 S) domain, the C-terminal (NC-1) domain, or the major triple-helical region between the terminal domains. Degradation of collagen IV was extensive; many fragments were found, from both GBM and LBM, in the M(r) range 25,000-380,000. A large fragment of laminin (M(r) > 380,000) was found in the GBM digests without reduction, but it dissociated into 220,000-M(r) chains upon reduction. The results suggest that MMP-3 plays an important role in the catabolism of basement membranes.

Original languageEnglish (US)
Pages (from-to)413-419
Number of pages7
JournalBiochemical Journal
Volume256
Issue number2
DOIs
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Bejarano, P. A., Noelken, M. E., Suzuki, K., Hudson, B. G., & Nagase, H. (1988). Degradation of basement membranes by human matrix metalloproteinase 3 (stromelysin). Biochemical Journal, 256(2), 413-419. https://doi.org/10.1042/bj2560413