Deficient T H-1 responses from TNF-a-matured and α-CD40-matured dendritic cells

William K. Decker, Sufang Li, Dongxia Xing, Simon N. Robinson, Hong Yang, David Steiner, Krishna V. Komanduri, Catherine M. Bollard, Elizabeth J. Shpall

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The ultimate success of dendritic cell (DC) vaccination for the active immunotherapy of neoplasia is thought to be dependent on a very large number of variables, including DC generation protocol, loading methodology, dose, route of administration, and maturation method. Although the use of a maturation cocktail comprising interleukin (IL)-1β, tumor necrosis factor-α, IL-6, and prostaglandin E 2 (ITIP) has recently appeared in the literature, much of the data in the basic and clinical literature have been generated using DCs matured with the single inflammatory cytokine TNF-α. Here, we demonstrate that DCs matured with TNF-α alone or in combination with CD40 agonism are highly deficient, both physiologically and functionally, in comparison with DCs matured with IL-1β, TNF-α, IL-6, and prostaglandin E 2. Empirically, the data suggest that DCs matured with these agents are deficient in the induction of type 1 T-helper responses. We further speculate that DCs matured by these methods might be suboptimal for the priming of naive responses.

Original languageEnglish (US)
Pages (from-to)157-165
Number of pages9
JournalJournal of Immunotherapy
Volume31
Issue number2
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

Keywords

  • Dendritic cell
  • Maturation
  • T -1 response

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Cancer Research
  • Pharmacology

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    Decker, W. K., Li, S., Xing, D., Robinson, S. N., Yang, H., Steiner, D., Komanduri, K. V., Bollard, C. M., & Shpall, E. J. (2008). Deficient T H-1 responses from TNF-a-matured and α-CD40-matured dendritic cells. Journal of Immunotherapy, 31(2), 157-165. https://doi.org/10.1097/CJI.0b013e31815eb0df