Deficient expression in multiple sclerosis of the inhibitory transcription factor Sp3 in mononuclear blood cells

Maria C. Grekova, Eve D. Robinson, Marc A. Faerber, Paul Katz, Henry F. McFarland, John R. Richert

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

To evaluate differential gene expression in multiple sclerosis (MS) patients and control subjects, we used differential display to screen for messenger RNAs that are differentially expressed in peripheral blood mononuclear cells from monozygotic twins who are discordant for MS. We identified a 232-bp complementary DNA fragment, present only in material from the normal twin, that exhibited 100% identity with the inhibitory transcription factor Sp3. Oligonucleotide primers corresponding to Sp3 messenger RNA sequences amplified complementary DNA of appropriate size from 83% of control subjects but from only 21% of MS patients (p < 0.001). These results suggest that Sp3 gene transcription is suppressed in peripheral blood mononuclear cells from most MS patients and that other genes whose expression is normally suppressed by Sp3 in immune cells may consequently be overexpressed.

Original languageEnglish (US)
Pages (from-to)108-112
Number of pages5
JournalAnnals of neurology
Volume40
Issue number1
DOIs
StatePublished - Jul 1 1996

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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    Grekova, M. C., Robinson, E. D., Faerber, M. A., Katz, P., McFarland, H. F., & Richert, J. R. (1996). Deficient expression in multiple sclerosis of the inhibitory transcription factor Sp3 in mononuclear blood cells. Annals of neurology, 40(1), 108-112. https://doi.org/10.1002/ana.410400117