Deficient DNA repair in amyotrophic lateral sclerosis cells

R. Tandan, S. H. Robison, J. Scott Munzer, Walter G. Bradley

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We studied survival and DNA repair capacity in cultured sporadic ALS and control skin fibroblasts after treatment with DNA damaging agents producing different types of lesions. Mean survival in ALS and control fibroblasts was similar after exposure to ultraviolet (UV) light, x-rays and mitomycin C (MMC). Both mean survival and mean unscheduled (repair) DNA synthesis (UDS) were significantly reduced in ALS fibroblasts following treatment with the alkylating agent methyl methane sulfonate (MMS). These data suggest that ALS cells are relatively deficient in the repair of alkylation damage, possibly of apurinic/apyrimidinic sites, and that they are not unduly sensitive to DNA damage produced by UV light, x-rays and MMC. Normal survival and UDS seen in some patients' cells after MMS treatment indicate a spectrum of repair efficiency, and suggest heterogeneity of the biochemical defect in ALS.

Original languageEnglish (US)
Pages (from-to)189-203
Number of pages15
JournalJournal of the Neurological Sciences
Volume79
Issue number1-2
DOIs
StatePublished - Jun 1987
Externally publishedYes

Keywords

  • Alkylating agent
  • Amyotrophic lateral sclerosis
  • Cell survival
  • Deoxyribonucleic acid repair
  • Fibroblasts
  • Methyl methane sulfonate
  • Unscheduled deoxyribonucleic acid synthesis

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

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