TY - JOUR
T1 - Decyl methacrylate-based microspot optodes
AU - Watts, Amanda S.
AU - Urbas, Aaron A.
AU - Finley, Timothy
AU - Gavalas, Vasilis G.
AU - Bachas, Leonidas G.
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/1/15
Y1 - 2006/1/15
N2 - Optode sensing membranes employing decyl methacrylate cross-linked with 1,6-hexanediol dimethacrylate as the polymer support were fabricated by a direct microspotting method on several surfaces. Photopolymerization was used to attach the microspots to the substrate. Using this method, diameters in the micrometer domain were obtained. Silanized glass, poly(methyl methacrylate) (PMMA), polycarbonate, and poly(dimethylsiloxane) were tested as possible substrates. Both polypropylene tips and the steel tips of drafting pens were used for spotting. It was determined that both silanized glass and PMMA gave working optodes, but the ones on PMMA did not fit the theoretical model. Diameters of 994 ± 80 and 1279 ± 85 μm were obtained on silanized glass and PMMA, respectively, using the polypropylene tips for spotting. Different size optodes were fabricated using 0.35- and 0.50-mm steel drafting pen tips. The 0.35-mm tips produced diameters of 895 ± 26 and 688 ± 54 μm on silanized glass and PMMA, respectively, and the 0.50-mm tips produced diameters of 1274 ± 94 μm on silanized glass and 839 ± 28 μm on PMMA. Thus, the microspot size can be controlled based on the hydrophobicity of the surface and the size of the tip used for spotting. Calibration plots of potassium optode microspots indicated that miniaturization does not alter response characteristics, such as selectivity, response time, and dynamic range, of the optodes.
AB - Optode sensing membranes employing decyl methacrylate cross-linked with 1,6-hexanediol dimethacrylate as the polymer support were fabricated by a direct microspotting method on several surfaces. Photopolymerization was used to attach the microspots to the substrate. Using this method, diameters in the micrometer domain were obtained. Silanized glass, poly(methyl methacrylate) (PMMA), polycarbonate, and poly(dimethylsiloxane) were tested as possible substrates. Both polypropylene tips and the steel tips of drafting pens were used for spotting. It was determined that both silanized glass and PMMA gave working optodes, but the ones on PMMA did not fit the theoretical model. Diameters of 994 ± 80 and 1279 ± 85 μm were obtained on silanized glass and PMMA, respectively, using the polypropylene tips for spotting. Different size optodes were fabricated using 0.35- and 0.50-mm steel drafting pen tips. The 0.35-mm tips produced diameters of 895 ± 26 and 688 ± 54 μm on silanized glass and PMMA, respectively, and the 0.50-mm tips produced diameters of 1274 ± 94 μm on silanized glass and 839 ± 28 μm on PMMA. Thus, the microspot size can be controlled based on the hydrophobicity of the surface and the size of the tip used for spotting. Calibration plots of potassium optode microspots indicated that miniaturization does not alter response characteristics, such as selectivity, response time, and dynamic range, of the optodes.
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U2 - 10.1021/ac051652e
DO - 10.1021/ac051652e
M3 - Article
C2 - 16408935
AN - SCOPUS:30744446484
VL - 78
SP - 524
EP - 529
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 2
ER -