Decoration of actin filaments with skeletal muscle heavy meromyosin containing either phosphorylated or dephosphorylated regulatory light chains

D. Stȩpkowski, H. Osińska, D. Szczȩsna, M. Wrotek, I. Ka̧kol

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Heavy meromyosin containing almost intact regulatory light chains (LC2) was obtained from monomeric phosphorylated and dephosphorylated rabbit fast skeletal muscle myosin by brief chymotryptic digestion in the presence of CaCl2. Actin filaments, complexed with heavy meromyosin display two different forms of arrowhead, depending on the form of LC2.

Original languageEnglish (US)
Pages (from-to)337-340
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume830
Issue number3
DOIs
StatePublished - Aug 23 1985

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Keywords

  • (Rabbit skeletal muscle)
  • Decorated actin filament
  • Regulatory light chain dephosphorylation
  • Regulatory light chain phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology

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