Decoding molecular and cellular heterogeneity of mouse nucleus accumbens

Renchao Chen, Timothy R. Blosser, Mohamed N. Djekidel, Junjie Hao, Aritra Bhattacherjee, Wenqiang Chen, Luis M. Tuesta, Xiaowei Zhuang, Yi Zhang

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The nucleus accumbens (NAc) plays an important role in regulating multiple behaviors, and its dysfunction has been linked to many neural disorders. However, the molecular, cellular and anatomic heterogeneity underlying its functional diversity remains incompletely understood. In this study, we generated a cell census of the mouse NAc using single-cell RNA sequencing and multiplexed error-robust fluorescence in situ hybridization, revealing a high level of cell heterogeneity in this brain region. Here we show that the transcriptional and spatial diversity of neuron subtypes underlie the NAc’s anatomic and functional heterogeneity. These findings explain how the seemingly simple neuronal composition of the NAc achieves its highly heterogenous structure and diverse functions. Collectively, our study generates a spatially resolved cell taxonomy for understanding the structure and function of the NAc, which demonstrates the importance of combining molecular and spatial information in revealing the fundamental features of the nervous system.

Original languageEnglish (US)
Pages (from-to)1757-1771
Number of pages15
JournalNature Neuroscience
Volume24
Issue number12
DOIs
StatePublished - Dec 2021
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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