Decidual macrophages are the population of decidual adherent cells which regulates perforin expression in cytolytic cells

V. Sotošek, G. Laškarin, N. Štrbo, G. Dohr, A. Blaschitz, E. R. Podack, Daniel Rukavina

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

PROBLEM: We have shown that addition of decidual adherent cells (DAC) to the culture of decidual lymphocytes (DL) prevents the downregulation of perforin expression in these cells. Because DAC are a mixture of various cell populations, the aim is to analyze immunophenotypic characteristics of DAC and to determine which cell population is involved in the regulation of perforin expression. METHOD OF STUDY: First trimester pregnancy decidual cells were obtained by enzymatic tissue digestion. Decidual cells and peripheral blood lymphocytes (PBL) were centrifuged on Ficoll-Hypaque density gradient and cultured overnight to obtain adherent cells, which were analyzed by flow cytometry and immunocytochemically. RESULTS: Almost all peripheral blood adherent cells (PBAC) (ca 90%) expressed monocyte/macrophage markers but only 10-20% of DAC. The rest of DAC expressed markers of stromal cells. HLA-DR depleted population of DAC (stromal cells only) could not prevent downregulation of perforin in expression in cultured DL and PBL. CONCLUSION: Decidual macrophages are involved in the regulation of perforin expression in DL.

Original languageEnglish (US)
Pages (from-to)76-82
Number of pages7
JournalAmerican Journal of Reproductive Immunology
Volume42
Issue number2
StatePublished - Aug 1 1999

Keywords

  • Decidual macrophages
  • First trimester pregnancy
  • Perforin

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

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