Death signal-induced localization of p53 protein to mitochondria

A potential role in apoptotic signaling

Natalie D. Marchenko, Alexander Zaika, Ute M. Moll

Research output: Contribution to journalArticle

724 Citations (Scopus)

Abstract

The mechanism of p53-mediated apoptosis after cellular stress remains poorly understood. Evidence suggests that p53 induces cell death by a multitude of molecular pathways involving activation of target genes and transcriptionally independent direct signaling. Mitochondria play a key role in apoptosis. We show here that a fraction of p53 protein localizes to mitochondria at the onset of p53-dependent apoptosis but not during p53- independent apoptosis or p53-mediated cell cycle arrest. The accumulation of p53 to mitochondria is rapid (within 1 h after p53 activation) and precedes changes in mitochondrial membrane potential, cytochrome c release, and procaspase-3 activation. Immunoelectron microscopy and immunofluorescence- activated cell sorter analysis of isolated mitochondria show that the majority of mitochondrial p53 localizes to the membranous compartment, whereas a fraction is found in a complex with the mitochondrial import motor mt hsp70. After induction of ectopic p53 without additional DNA damage in p53-deficient cells, p53 again partially localizes to mitochondria, preceding the onset of apoptosis. Overexpression of anti-apoptotic Bcl-2 or Bcl-xL abrogates stress signal-mediated mitochondrial p53 accumulation and apoptosis but not cell cycle arrest, suggesting a feedback signaling loop between p53 and mitochondrial apoptotic regulators. Importantly, bypassing the nucleus by targeting p53 to mitochondria using import leader fusions is sufficient to induce apoptosis in p53-deficient cells. We propose a model where p53 can contribute to apoptosis by direct signaling at the mitochondria, thereby amplifying the transcription-dependent apoptosis of p53.

Original languageEnglish (US)
Pages (from-to)16202-16212
Number of pages11
JournalJournal of Biological Chemistry
Volume275
Issue number21
DOIs
StatePublished - May 26 2000
Externally publishedYes

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Mitochondria
Apoptosis
Proteins
Chemical activation
Cell Cycle Checkpoints
Cells
Immunoelectron Microscopy
Mitochondrial Membrane Potential
Cell death
Transcription
Cytochromes c
Caspase 3
Transcriptional Activation
DNA Damage
Fluorescent Antibody Technique
Microscopic examination
Cell Death
Fusion reactions
Genes
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Death signal-induced localization of p53 protein to mitochondria : A potential role in apoptotic signaling. / Marchenko, Natalie D.; Zaika, Alexander; Moll, Ute M.

In: Journal of Biological Chemistry, Vol. 275, No. 21, 26.05.2000, p. 16202-16212.

Research output: Contribution to journalArticle

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