DEADSouth is a germ plasm specific DEAD-box RNA helicase in Xenopus related to eIF4A

Heather MacArthur; Douglas W. Houston; Mikhail Bubunenko; Luis Mosquera; Mary Lou King

doi:10.1016/S0925-4773(00)00357-9

DEADSouth is a germ plasm specific DEAD-box RNA helicase in Xenopus related to eIF4A

Heather MacArthur, Douglas W. Houston, Mikhail Bubunenko, Luis Mosquera, Mary Lou King

Cell Biology

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

DEADSouth was selected in a screen for localized RNAs in Xenopus oocytes. In situ hybridization analysis shows that DEADSouth localizes to the vegetal cortex via the mitochondrial cloud early in oogenesis and segregates with germ plasm during early embryogenesis. These results lend further support for the general concept that the role of the early RNA localization pathway in Xenopus is to localize germ cell components (reviewed in King, M.L., Zhou, Y., Bubunenko, M., 1999. BioEssays 21, 546-557). Further analysis shows that DEADSouth is a germline specific RNA, found exclusively within the germ plasm of oocytes and PGCs, as well as in male germ cells. Sequence comparisons with DEADSouth show it to be a member of a small sub-family of the DEAD-box RNA-dependent helicases related to eIF4A. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English (US)
Pages (from-to)	291-295
Number of pages	5
Journal	Mechanisms of Development
Volume	95
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(00)00357-9
State	Published - Jul 1 2000

Keywords

DEAD-box protein
eIF4A
Germ plasm
Localized RNA
RNA-binding helicase
Spermatogenesis
Xenopus

ASJC Scopus subject areas

Developmental Biology
Developmental Neuroscience

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title = "DEADSouth is a germ plasm specific DEAD-box RNA helicase in Xenopus related to eIF4A",

abstract = "DEADSouth was selected in a screen for localized RNAs in Xenopus oocytes. In situ hybridization analysis shows that DEADSouth localizes to the vegetal cortex via the mitochondrial cloud early in oogenesis and segregates with germ plasm during early embryogenesis. These results lend further support for the general concept that the role of the early RNA localization pathway in Xenopus is to localize germ cell components (reviewed in King, M.L., Zhou, Y., Bubunenko, M., 1999. BioEssays 21, 546-557). Further analysis shows that DEADSouth is a germline specific RNA, found exclusively within the germ plasm of oocytes and PGCs, as well as in male germ cells. Sequence comparisons with DEADSouth show it to be a member of a small sub-family of the DEAD-box RNA-dependent helicases related to eIF4A. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

keywords = "DEAD-box protein, eIF4A, Germ plasm, Localized RNA, RNA-binding helicase, Spermatogenesis, Xenopus",

author = "Heather MacArthur and Houston, {Douglas W.} and Mikhail Bubunenko and Luis Mosquera and King, {Mary Lou}",

note = "Funding Information: The complete coding portion of the DEADSouth sequence was generated by 5′RACE by Dr Zhentu Ying and combined with the LC1 sequence to produce a clone containing the complete coding sequence. We are grateful for the expert technical assistance provided by Elio Dancausse. This work was supported by grants from NIH (GM33932) and the Human Frontier Science Program to M.L. King.",

year = "2000",

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AU - MacArthur, Heather

AU - Houston, Douglas W.

AU - Bubunenko, Mikhail

AU - Mosquera, Luis

AU - King, Mary Lou

N1 - Funding Information: The complete coding portion of the DEADSouth sequence was generated by 5′RACE by Dr Zhentu Ying and combined with the LC1 sequence to produce a clone containing the complete coding sequence. We are grateful for the expert technical assistance provided by Elio Dancausse. This work was supported by grants from NIH (GM33932) and the Human Frontier Science Program to M.L. King.

PY - 2000/7/1

Y1 - 2000/7/1

N2 - DEADSouth was selected in a screen for localized RNAs in Xenopus oocytes. In situ hybridization analysis shows that DEADSouth localizes to the vegetal cortex via the mitochondrial cloud early in oogenesis and segregates with germ plasm during early embryogenesis. These results lend further support for the general concept that the role of the early RNA localization pathway in Xenopus is to localize germ cell components (reviewed in King, M.L., Zhou, Y., Bubunenko, M., 1999. BioEssays 21, 546-557). Further analysis shows that DEADSouth is a germline specific RNA, found exclusively within the germ plasm of oocytes and PGCs, as well as in male germ cells. Sequence comparisons with DEADSouth show it to be a member of a small sub-family of the DEAD-box RNA-dependent helicases related to eIF4A. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - DEADSouth was selected in a screen for localized RNAs in Xenopus oocytes. In situ hybridization analysis shows that DEADSouth localizes to the vegetal cortex via the mitochondrial cloud early in oogenesis and segregates with germ plasm during early embryogenesis. These results lend further support for the general concept that the role of the early RNA localization pathway in Xenopus is to localize germ cell components (reviewed in King, M.L., Zhou, Y., Bubunenko, M., 1999. BioEssays 21, 546-557). Further analysis shows that DEADSouth is a germline specific RNA, found exclusively within the germ plasm of oocytes and PGCs, as well as in male germ cells. Sequence comparisons with DEADSouth show it to be a member of a small sub-family of the DEAD-box RNA-dependent helicases related to eIF4A. Copyright (C) 2000 Elsevier Science Ireland Ltd.

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KW - RNA-binding helicase

KW - Spermatogenesis

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