Abstract
We have cloned and sequenced a new gene from Escherichia coli which encodes a 64-kDa protein. The inferred amino acid sequence of the protein shows remarkable similarity to eIF4A, a murine translation initiation factor that has an ATP-dependent RNA helicase activity and is a founding member of the D-E-A-D family of proteins (characterized by a conserved Asp-Glu-Ala-Asp motif). Our new gene, called deaD, was cloned as a gene dosage-dependent suppressor of temperature-sensitive mutations in rpsB, the gene encoding ribosomal protein S2. We suggest that the DeaD protein plays a hitherto unknown role in translation in E. coli.
Original language | English (US) |
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Pages (from-to) | 3291-3302 |
Number of pages | 12 |
Journal | Journal of bacteriology |
Volume | 173 |
Issue number | 11 |
DOIs | |
State | Published - 1991 |
ASJC Scopus subject areas
- Microbiology
- Molecular Biology