deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2

W. M. Toone, K. E. Rudd, J. D. Friesen

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

We have cloned and sequenced a new gene from Escherichia coli which encodes a 64-kDa protein. The inferred amino acid sequence of the protein shows remarkable similarity to eIF4A, a murine translation initiation factor that has an ATP-dependent RNA helicase activity and is a founding member of the D-E-A-D family of proteins (characterized by a conserved Asp-Glu-Ala-Asp motif). Our new gene, called deaD, was cloned as a gene dosage-dependent suppressor of temperature-sensitive mutations in rpsB, the gene encoding ribosomal protein S2. We suggest that the DeaD protein plays a hitherto unknown role in translation in E. coli.

Original languageEnglish (US)
Pages (from-to)3291-3302
Number of pages12
JournalJournal of bacteriology
Volume173
Issue number11
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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