De novo reconstitution of a functional mammalian urinary bladder by tissue engineering

Frank Oberpenning, Jun Meng, James J. Yoo, Anthony Atala

Research output: Contribution to journalArticlepeer-review

673 Scopus citations


Human organ replacement is limited by a donor shortage, problems with tissue compatibility, and rejection. Creation of an organ with autologous tissue would be advantageous. In this study, transplantable urinary bladder neo-organs were reproducibly created in vitro from urothelial and smooth muscle cells grown in culture from canine native bladder biopsies and seeded onto preformed bladder-shaped polymers. The native bladders were subsequently excised from canine donors and replaced with the tissue-engineered neo- organs. In functional evaluations for up to 11 months, the bladder neo- organs demonstrated a normal capacity to retain urine, normal elastic properties, and histologic architecture. This study demonstrates, for the first time, that successful reconstitution of an autonomous hollow organ is possible using tissue-engineering methods.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalNature Biotechnology
Issue number2
StatePublished - Feb 1999
Externally publishedYes


  • Augmentation
  • Cell culture
  • Cystectomy
  • Cystoplasty
  • Neo-bladder

ASJC Scopus subject areas

  • Microbiology


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