DARPP-32 and inhibitor-1 are expressed in pancreatic β-cells

Lena Lilja, Björn Meister, Per Olof Berggren, Christina Bark

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Insulin secretion from pancreatic β-cells has to be tightly regulated to ensure accurate glucose homeostasis. The capacity of β-cells to respond to extracellular stimulation is determined by several signaling pathways. One important feature of these pathways is phosphorylation and subsequent dephosphorylation of a wide range of cellular substrates. Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine protein phosphatase that controls a multitude of physiological processes. We have investigated the expression and cellular distribution of two endogenous inhibitors of PP1 activity in β-cells. RT-PCR, Western blotting, and immunohistochemistry showed that DARPP-32 and inhibitor-1 are present in insulin-secreting endocrine β-cells. Subcellular fractionation of mouse islets revealed that both PP1 inhibitors predominantly localized to cytosol-enriched fractions. Inhibitor-1 was also present in fractions containing plasma membrane-associated proteins. These data indicate a potential role for DARPP-32 and inhibitor-1 in the regulation of PP1 activity in pancreatic β-cell stimulus-secretion coupling.

Original languageEnglish
Pages (from-to)673-677
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume329
Issue number2
DOIs
StatePublished - Apr 8 2005
Externally publishedYes

Fingerprint

Protein Phosphatase 1
Insulin
Phosphorylation
Phosphoprotein Phosphatases
Cell membranes
Fractionation
Membrane Proteins
Physiological Phenomena
Glucose
Endocrine Cells
Insulin-Secreting Cells
Substrates
Cytosol
Blood Proteins
Homeostasis
Western Blotting
Immunohistochemistry
Cell Membrane
Polymerase Chain Reaction
protein phosphatase inhibitor-1

Keywords

  • Dephosphorylation
  • Exocytosis
  • Immunohistochemistry
  • Insulin
  • Phosphatase
  • PP1 inhibitor
  • RT-PCR
  • Secretion
  • Subcellular fractionation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

DARPP-32 and inhibitor-1 are expressed in pancreatic β-cells. / Lilja, Lena; Meister, Björn; Berggren, Per Olof; Bark, Christina.

In: Biochemical and Biophysical Research Communications, Vol. 329, No. 2, 08.04.2005, p. 673-677.

Research output: Contribution to journalArticle

Lilja, Lena ; Meister, Björn ; Berggren, Per Olof ; Bark, Christina. / DARPP-32 and inhibitor-1 are expressed in pancreatic β-cells. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 329, No. 2. pp. 673-677.
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