“Dark” carbon dots specifically “light-up” calcified zebrafish bones

Shanghao Li, Isaac Skromne, Zhili Peng, Julia Dallman, Abdulrahman O. Al-Youbi, Abdulaziz S. Bashammakh, Mohammad S. El-Shahawi, Roger Leblanc

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Because accidents, disease and aging compromise the structural and physiological functions of bones, the development of an in vivo bone imaging test is critical to identify, detect and diagnose bone related development and dysfunctions. Recent advances in fluorescence instrumentation offer a new alternative for traditional bone imaging methods. However, the development of new in vivo bone imaging fluorescence materials has significantly lagged behind. Here we show that carbon dot nanoparticles (C-dots) with low quantum yield (“dark”) bind to calcified bone structures of live zebrafish larvae with high affinity and selectivity. Binding resulted in a strong enhancement of luminescence that was not observed in other tissues, including non-calcified endochondral elements. Retention of C-dots by bones was very stable, long lasting, and with no detectable toxicity. Furthermore, we found C-dots to be a suitable carrier to deliver fluorescein to bones. These observations support a novel and revolutionary use of C-dots as highly specific bioagents for bone imaging and diagnosis, and as bone-specific drug delivery vehicles.

Original languageEnglish (US)
Pages (from-to)7398-7405
Number of pages8
JournalJournal of Materials Chemistry B
Volume4
Issue number46
DOIs
StatePublished - 2016

Fingerprint

Zebrafish
Bone
Carbon
Light
Bone and Bones
Nanoparticles
Bone Development
Imaging techniques
Fluorescence
Optical Imaging
Luminescence
Fluorescein
Accidents
Larva
Quantum yield
Drug delivery
Chemical elements
Toxicity
Aging of materials
Tissue

ASJC Scopus subject areas

  • Chemistry(all)
  • Medicine(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

“Dark” carbon dots specifically “light-up” calcified zebrafish bones. / Li, Shanghao; Skromne, Isaac; Peng, Zhili; Dallman, Julia; Al-Youbi, Abdulrahman O.; Bashammakh, Abdulaziz S.; El-Shahawi, Mohammad S.; Leblanc, Roger.

In: Journal of Materials Chemistry B, Vol. 4, No. 46, 2016, p. 7398-7405.

Research output: Contribution to journalArticle

Li, S, Skromne, I, Peng, Z, Dallman, J, Al-Youbi, AO, Bashammakh, AS, El-Shahawi, MS & Leblanc, R 2016, '“Dark” carbon dots specifically “light-up” calcified zebrafish bones', Journal of Materials Chemistry B, vol. 4, no. 46, pp. 7398-7405. https://doi.org/10.1039/c6tb02241c
Li, Shanghao ; Skromne, Isaac ; Peng, Zhili ; Dallman, Julia ; Al-Youbi, Abdulrahman O. ; Bashammakh, Abdulaziz S. ; El-Shahawi, Mohammad S. ; Leblanc, Roger. / “Dark” carbon dots specifically “light-up” calcified zebrafish bones. In: Journal of Materials Chemistry B. 2016 ; Vol. 4, No. 46. pp. 7398-7405.
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