DAPI fluorescence in nuclei isolated from tumors

Awtar Krishan, Payai D. Dandekar

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

In DNA histograms of some human solid tumors stained with nuclear isolation medium-4,6-diamidino-2-phenylindole dihydrochloride (NIM-DAPI), the coefficient of variation (CV) of the G0/G1 peak was broad, and in nuclear volume vs DNA scattergrams, a prominent slope was seen. To determine the cause for this, nuclei from frozen breast tumors were stained with NIM-DAPI and analyzed after dilution or resuspension in PBS. In two-color (blue vs red) analysis, most of the slope and broad CV was due to red fluorescence of nuclei stained with NIM-DAPI, which was reduced on dilution or resuspension in PBS, resulting in elimination of the slope and tightening of the CV.

Original languageEnglish (US)
Pages (from-to)1033-1036
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume53
Issue number8
DOIs
StatePublished - Aug 2005

Keywords

  • DAPI
  • DNA content
  • Flow cytometry
  • Nuclear volume
  • Tumors

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

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