Cytotoxic and photodynamic effects of photofrin® on sensitive and multi-drug-resistant friend leukaemia cells

M. Dellinger, G. Moreno, C. Salet, H. Tapiero, Theodore Lampidis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

To study cross-resistance to Photofrin® (PF) photosensitization, a Friend leukaemia cell line (ADM-RFLC) with a high level of multi-drug resistance (MDR) and the parental sensitive cell line (FLC) have been used. PF uptake measured by HPLC shows a similar intracellular drug accumulation in both cell lines. The ID50s for cell growth inhibition by PF are also similar after exposure in the dark in the two cell lines, while after illumination they are slightly lower in ADM-RFLC than in FLC cells. Moreover, verapamil, known to reverse the MDR phenotype induced by P-glycoprotein over-expression (the drug efflux mechanism), affects equally ADM-RFLC and FLC cells sensitivity to PF. In addition, photodynamic treatment with PF did not reverse the resistance to rhodamine 123 and aclarubicin, but partly reverses resistance of ADM-RFLC cells to antitubulin drugs such as vinblastine or vincristine. These latter results could have clinical application in the treatment of tumours expressing the MDR phenotype.

Original languageEnglish
Pages (from-to)735-741
Number of pages7
JournalInternational Journal of Radiation Biology
Volume62
Issue number6
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Dihematoporphyrin Ether
Delta modulation
leukemias
leukemia
multiple drug resistance
Leukemia
drugs
Cells
cell lines
cultured cells
Multiple Drug Resistance
Cell Line
cells
Pharmaceutical Preparations
phenotype
vinblastine
vincristine
Glycoproteins
Aclarubicin
verapamil

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Cytotoxic and photodynamic effects of photofrin® on sensitive and multi-drug-resistant friend leukaemia cells. / Dellinger, M.; Moreno, G.; Salet, C.; Tapiero, H.; Lampidis, Theodore.

In: International Journal of Radiation Biology, Vol. 62, No. 6, 01.01.1992, p. 735-741.

Research output: Contribution to journalArticle

@article{16831ca3dad546d4ad8d452d73d66b7b,
title = "Cytotoxic and photodynamic effects of photofrin{\circledR} on sensitive and multi-drug-resistant friend leukaemia cells",
abstract = "To study cross-resistance to Photofrin{\circledR} (PF) photosensitization, a Friend leukaemia cell line (ADM-RFLC) with a high level of multi-drug resistance (MDR) and the parental sensitive cell line (FLC) have been used. PF uptake measured by HPLC shows a similar intracellular drug accumulation in both cell lines. The ID50s for cell growth inhibition by PF are also similar after exposure in the dark in the two cell lines, while after illumination they are slightly lower in ADM-RFLC than in FLC cells. Moreover, verapamil, known to reverse the MDR phenotype induced by P-glycoprotein over-expression (the drug efflux mechanism), affects equally ADM-RFLC and FLC cells sensitivity to PF. In addition, photodynamic treatment with PF did not reverse the resistance to rhodamine 123 and aclarubicin, but partly reverses resistance of ADM-RFLC cells to antitubulin drugs such as vinblastine or vincristine. These latter results could have clinical application in the treatment of tumours expressing the MDR phenotype.",
author = "M. Dellinger and G. Moreno and C. Salet and H. Tapiero and Theodore Lampidis",
year = "1992",
month = "1",
day = "1",
doi = "10.1080/09553009214552691",
language = "English",
volume = "62",
pages = "735--741",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Informa Healthcare",
number = "6",

}

TY - JOUR

T1 - Cytotoxic and photodynamic effects of photofrin® on sensitive and multi-drug-resistant friend leukaemia cells

AU - Dellinger, M.

AU - Moreno, G.

AU - Salet, C.

AU - Tapiero, H.

AU - Lampidis, Theodore

PY - 1992/1/1

Y1 - 1992/1/1

N2 - To study cross-resistance to Photofrin® (PF) photosensitization, a Friend leukaemia cell line (ADM-RFLC) with a high level of multi-drug resistance (MDR) and the parental sensitive cell line (FLC) have been used. PF uptake measured by HPLC shows a similar intracellular drug accumulation in both cell lines. The ID50s for cell growth inhibition by PF are also similar after exposure in the dark in the two cell lines, while after illumination they are slightly lower in ADM-RFLC than in FLC cells. Moreover, verapamil, known to reverse the MDR phenotype induced by P-glycoprotein over-expression (the drug efflux mechanism), affects equally ADM-RFLC and FLC cells sensitivity to PF. In addition, photodynamic treatment with PF did not reverse the resistance to rhodamine 123 and aclarubicin, but partly reverses resistance of ADM-RFLC cells to antitubulin drugs such as vinblastine or vincristine. These latter results could have clinical application in the treatment of tumours expressing the MDR phenotype.

AB - To study cross-resistance to Photofrin® (PF) photosensitization, a Friend leukaemia cell line (ADM-RFLC) with a high level of multi-drug resistance (MDR) and the parental sensitive cell line (FLC) have been used. PF uptake measured by HPLC shows a similar intracellular drug accumulation in both cell lines. The ID50s for cell growth inhibition by PF are also similar after exposure in the dark in the two cell lines, while after illumination they are slightly lower in ADM-RFLC than in FLC cells. Moreover, verapamil, known to reverse the MDR phenotype induced by P-glycoprotein over-expression (the drug efflux mechanism), affects equally ADM-RFLC and FLC cells sensitivity to PF. In addition, photodynamic treatment with PF did not reverse the resistance to rhodamine 123 and aclarubicin, but partly reverses resistance of ADM-RFLC cells to antitubulin drugs such as vinblastine or vincristine. These latter results could have clinical application in the treatment of tumours expressing the MDR phenotype.

UR - http://www.scopus.com/inward/record.url?scp=0027053108&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027053108&partnerID=8YFLogxK

U2 - 10.1080/09553009214552691

DO - 10.1080/09553009214552691

M3 - Article

C2 - 1362767

AN - SCOPUS:0027053108

VL - 62

SP - 735

EP - 741

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 6

ER -