TY - JOUR
T1 - Cytoskeletal actin microfilaments and the transient outward potassium current in hypertrophied rat ventriculocytes
AU - Yang, Xiangjun
AU - Pedro, J. I.
AU - Pham, Thai V.
AU - Wasserlauf, Bernard J.
AU - Smets, Marcel J.D.
AU - Myerburg, Robert J.
AU - Gelband, Henry
AU - Hoffman, Brian F.
AU - Bassett, Arthur L.
PY - 2002/6/1
Y1 - 2002/6/1
N2 - The durations of transmembrane action potentials recorded from single myocytes isolated from the endocardial surface of hypertrophied left ventricles of rats were increased, compared to the durations recorded from normal left ventricular cells at 36-37°C. Exposure to phalloidin (1-20 μM, < 20 min), a specific stabilizer of the non-myofibrillar actin microfilament component of the cardiac cytoskeleton, had no effect on action potential duration of normal cells, but significantly shortened the prolonged action potentials of hypertrophied cells. Cytochalasin D (5-50 μM), a disrupter of the actin microfilaments, also had little effect on action potential duration of normal cells. However, cytochalasin D further increased the action potential duration of hypertrophied cells at 10 min exposure. The addition of phalloidin to solutions containing cytochalasin D, reduced the latter's increase of action potential duration in hypertrophied cells. Whole-cell transient outward K+ current (Itol) density was significantly decreased in hypertrophied cells. At a test potential of +60 mV, the mean Itol density recorded from normal cells was 13.5 ± 1.1 pA pF-1 (n = 18) compared to 4.17 ± 1.2 pA pF-1 for LVH cells (n = 22; P < 0.05). Phalloidin (20 μM) increased and cytochalasin D (50 μM) decreased whole-cell Itol in hypertrophied cells but had no effect on Itol in normal cells. When equimolar concentrations were used, phalloidin, 10 μM, reversed the decrease in Itol brought about by cytochalasin D, 10 μM, in hypertrophied cells. The L-type calcium current density was reduced in LVH compared to normal cells. Phalloidin (20 μM) and cytochalasin D (50 μM) had no effect on ICa,L in normal or LVH myocytes. The decrease in Itol in hypertrophied cells and the altered Itol responsiveness to phalloidin and cytochalasin D reflect modification of Itol channel function mediated, in part, through hypertrophy-altered cytoskeletal actin microfilament regulation of Itol.
AB - The durations of transmembrane action potentials recorded from single myocytes isolated from the endocardial surface of hypertrophied left ventricles of rats were increased, compared to the durations recorded from normal left ventricular cells at 36-37°C. Exposure to phalloidin (1-20 μM, < 20 min), a specific stabilizer of the non-myofibrillar actin microfilament component of the cardiac cytoskeleton, had no effect on action potential duration of normal cells, but significantly shortened the prolonged action potentials of hypertrophied cells. Cytochalasin D (5-50 μM), a disrupter of the actin microfilaments, also had little effect on action potential duration of normal cells. However, cytochalasin D further increased the action potential duration of hypertrophied cells at 10 min exposure. The addition of phalloidin to solutions containing cytochalasin D, reduced the latter's increase of action potential duration in hypertrophied cells. Whole-cell transient outward K+ current (Itol) density was significantly decreased in hypertrophied cells. At a test potential of +60 mV, the mean Itol density recorded from normal cells was 13.5 ± 1.1 pA pF-1 (n = 18) compared to 4.17 ± 1.2 pA pF-1 for LVH cells (n = 22; P < 0.05). Phalloidin (20 μM) increased and cytochalasin D (50 μM) decreased whole-cell Itol in hypertrophied cells but had no effect on Itol in normal cells. When equimolar concentrations were used, phalloidin, 10 μM, reversed the decrease in Itol brought about by cytochalasin D, 10 μM, in hypertrophied cells. The L-type calcium current density was reduced in LVH compared to normal cells. Phalloidin (20 μM) and cytochalasin D (50 μM) had no effect on ICa,L in normal or LVH myocytes. The decrease in Itol in hypertrophied cells and the altered Itol responsiveness to phalloidin and cytochalasin D reflect modification of Itol channel function mediated, in part, through hypertrophy-altered cytoskeletal actin microfilament regulation of Itol.
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U2 - 10.1113/jphysiol.2002.019562
DO - 10.1113/jphysiol.2002.019562
M3 - Article
C2 - 12042348
AN - SCOPUS:0036623843
VL - 541
SP - 411
EP - 421
JO - Journal of Physiology
JF - Journal of Physiology
SN - 0022-3751
IS - 2
ER -