Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II

Coleen M Atkins, Naohito Nozaki, Yasushi Shigeri, Thomas R. Soderling

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Phosphorylation of cytoplasmic polyadenylation element binding protein (CPEB) regulates protein synthesis in hippocampal dendrites. CPEB binds the 3′ untranslated region (UTR) of cytoplasmic mRNAs and, when phosphorylated, initiates mRNA polyadenylation and translation. We report that, of the protein kinases activated in the hippocampus during synaptic plasticity, calcium/calmodulin-dependent protein kinase II (CaMKII) robustly phosphorylated the regulatory site (threonine 171) in CPEB in vitro. In postsynaptic density fractions or hippocampal neurons, CPEB phosphorylation increased when CaMKII was activated. These increases in CPEB phosphorylation were attenuated by a specific peptide inhibitor of CaMKII and by the general CaM-kinase inhibitor KN-93. Inhibitors of protein phosphatase 1 increased basal CPEB phosphorylation in neurons; this was also attenuated by a CaM-kinase inhibitor. To determine whether CaM-kinase activity regulates CPEB-dependent mRNA translation, hippocampal neurons were transfected with luciferase fused to a 3′ UTR containing CPE-binding elements. Depolarization of neurons stimulated synthesis of luciferase; this was abrogated by inhibitors of protein synthesis, mRNA polyadenylation, and CaMKII. These results demonstrate that CPEB phosphorylation and translation are regulated by CaMKII activity and provide a possible mechanism for how dendritic protein synthesis in the hippocampus may be stimulated during synaptic plasticity.

Original languageEnglish
Pages (from-to)5193-5201
Number of pages9
JournalJournal of Neuroscience
Volume24
Issue number22
DOIs
StatePublished - Jun 2 2004
Externally publishedYes

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases
Polyadenylation
Carrier Proteins
Proteins
Phosphorylation
Protein Biosynthesis
Neurons
Neuronal Plasticity
Phosphotransferases
3' Untranslated Regions
Luciferases
Hippocampus
Post-Synaptic Density
Messenger RNA
Protein Synthesis Inhibitors
Threonine
Dendrites
Protein Kinases

Keywords

  • CaMKII
  • CPEB
  • Polyadenylation
  • Protein phosphatase
  • Protein synthesis
  • Synaptic plasticity
  • Translation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cytoplasmic polyadenylation element binding protein-dependent protein synthesis is regulated by calcium/calmodulin-dependent protein kinase II. / Atkins, Coleen M; Nozaki, Naohito; Shigeri, Yasushi; Soderling, Thomas R.

In: Journal of Neuroscience, Vol. 24, No. 22, 02.06.2004, p. 5193-5201.

Research output: Contribution to journalArticle

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