Cytoplasmic dynein participates in apically targeted stimulated secretory traffic in primary rabbit lacrimal acinar epithelial cells

Yanru Wang, Galina Jerdeva, Francie A. Yarber, Silvia R. da Costa, Jiansong Xie, Limin Qian, Chadron M. Rose, Constance Mazurek, Noriyuki Kasahara, Austin K. Mircheff, Sarah F. Hamm-Alvarez

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A major function of the acinar cells of the lacrimal gland is the production and stimulated release of tear proteins into ocular surface fluid. We investigate the participation of cytoplasmic dynein in carbachol-stimulated traffic to the apical plasma membrane in primary rabbit lacrimal acinar epithelial cells. Confocal fluorescence microscopy revealed a major carbachol-induced, microtubule-dependent recruitment of cytoplasmic dynein and the dynactin complex into the subapical region. Colocalization studies, sorbitol density gradient/phase partitioning analysis and microtubule-affinity purification of membranes showed that some dynein and dynactin complex were associated with VAMP2-enriched membranes. Adenovirus-mediated overexpression of p50/dynamitin inhibited the recruitment and colocalization of dynein, the dynactin complex and VAMP2 in the subapical region. Nocodazole treatment and p50/dynamitin overexpression also depleted subapical stores of rab3D in resting acini, suggesting that dynein activity was also involved in maintenance of rab3D-enriched secretory vesicles. These data implicate cytoplasmic dynein in stimulated traffic to the apical plasma membrane in these secretory epithelial cells.

Original languageEnglish (US)
Pages (from-to)2051-2065
Number of pages15
JournalJournal of Cell Science
Volume116
Issue number10
DOIs
StatePublished - May 15 2003
Externally publishedYes

Fingerprint

Cytoplasmic Dyneins
Acinar Cells
Tears
Dyneins
Epithelial Cells
Rabbits
Vesicle-Associated Membrane Protein 2
Carbachol
Microtubules
Cell Membrane
Nocodazole
Lacrimal Apparatus
Membranes
Sorbitol
Secretory Vesicles
Fluorescence Microscopy
Adenoviridae
Confocal Microscopy
Maintenance
Dynactin Complex

Keywords

  • Acinar secretion
  • Dynein
  • Exocytosis
  • Microtubule
  • rab3D
  • VAMP2

ASJC Scopus subject areas

  • Cell Biology

Cite this

Wang, Y., Jerdeva, G., Yarber, F. A., da Costa, S. R., Xie, J., Qian, L., ... Hamm-Alvarez, S. F. (2003). Cytoplasmic dynein participates in apically targeted stimulated secretory traffic in primary rabbit lacrimal acinar epithelial cells. Journal of Cell Science, 116(10), 2051-2065. https://doi.org/10.1242/jcs.00398

Cytoplasmic dynein participates in apically targeted stimulated secretory traffic in primary rabbit lacrimal acinar epithelial cells. / Wang, Yanru; Jerdeva, Galina; Yarber, Francie A.; da Costa, Silvia R.; Xie, Jiansong; Qian, Limin; Rose, Chadron M.; Mazurek, Constance; Kasahara, Noriyuki; Mircheff, Austin K.; Hamm-Alvarez, Sarah F.

In: Journal of Cell Science, Vol. 116, No. 10, 15.05.2003, p. 2051-2065.

Research output: Contribution to journalArticle

Wang, Y, Jerdeva, G, Yarber, FA, da Costa, SR, Xie, J, Qian, L, Rose, CM, Mazurek, C, Kasahara, N, Mircheff, AK & Hamm-Alvarez, SF 2003, 'Cytoplasmic dynein participates in apically targeted stimulated secretory traffic in primary rabbit lacrimal acinar epithelial cells', Journal of Cell Science, vol. 116, no. 10, pp. 2051-2065. https://doi.org/10.1242/jcs.00398
Wang, Yanru ; Jerdeva, Galina ; Yarber, Francie A. ; da Costa, Silvia R. ; Xie, Jiansong ; Qian, Limin ; Rose, Chadron M. ; Mazurek, Constance ; Kasahara, Noriyuki ; Mircheff, Austin K. ; Hamm-Alvarez, Sarah F. / Cytoplasmic dynein participates in apically targeted stimulated secretory traffic in primary rabbit lacrimal acinar epithelial cells. In: Journal of Cell Science. 2003 ; Vol. 116, No. 10. pp. 2051-2065.
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