Cytometric and electron microscopic studies of the direct interaction of divalent nickel with intact and chemically modified HuT-78 lymphoblasts

G. I. Malinin, F. J. Hornicek, H. K. Lo, Theodore Malinin

Research output: Contribution to journalArticle

Abstract

Cytometric and ultrastructural studies on 24 hr cultures of intact, 1.0 mM H5I06, and 0.1 mM SeO2-oxidized HuT-78 lymphoblasts were performed after their direct, 30 min interaction with 1.0 mM NiCl2. Except for moderately depressed cell viability, divalent nickel did not alter the progression of intact and oxidized target cells through the phases of the cell cycle. Although the plasma membrane remained structurally intact, marked distortion of mitochondria structure and increased osmiophilia were an invariable attribute of all nickel-pulsed cells. Moreover, numerous electron-opaque, intracellular depositions were detected in SeO2-oxidized, nickel-pulsed cells. It is concluded that the initial state of plasma membrane, and the interaction of nickel with other trace elements, have jointly determined the response of HuT-78 cells to brief and direct, divalent nickel pulses.

Original languageEnglish
Pages (from-to)27-41
Number of pages15
JournalCell Biology and Toxicology
Volume8
Issue number1
DOIs
StatePublished - Mar 1 1992

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Nickel
Electrons
Cell membranes
Cells
Cell Membrane
Mitochondria
Trace Elements
Cell Survival
Cell Cycle

Keywords

  • Cytometry
  • Lymphoblasts
  • Membrane
  • Nickel
  • Ultrastructure

ASJC Scopus subject areas

  • Toxicology
  • Cell Biology

Cite this

Cytometric and electron microscopic studies of the direct interaction of divalent nickel with intact and chemically modified HuT-78 lymphoblasts. / Malinin, G. I.; Hornicek, F. J.; Lo, H. K.; Malinin, Theodore.

In: Cell Biology and Toxicology, Vol. 8, No. 1, 01.03.1992, p. 27-41.

Research output: Contribution to journalArticle

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