TY - JOUR
T1 - Cytokinetic and cytogenetic effects of some agricultural chemicals on human lymphoid cells in vitro
T2 - organophosphates
AU - Sobti, R. C.
AU - Krishan, A.
AU - Pfaffenberger, C. D.
N1 - Funding Information:
* Supported by USPHS Grant CA-14395 and ACS InsUtut~onal Grant IU-51U-01 ** To whom reprint requests may be sent at Cancer Center, R-71, P.O Box 016960, Mtarm, Florida 33101
PY - 1982
Y1 - 1982
N2 - In the present study, cytotoxic, cytostatic and cytogenetic effects of a number of organophosphate pesticides on human lymphoid cells (LAZ-007) in culture have been examined. Cytotoxic effects were dose related and often led to extensive cell kill. The pronounced effects of various organophosphates on the cell-cycle traverse were shown in data based on the enumeration of M1 and M3 metaphases after incubation of cells with BrdU. In cells incubated with 20 μg/ml of the various chemicals, the number of M1 metaphases ranged from 6% (R-1303) to 18% (Azodrin) compared to no M1 metaphases in control cultures. The number of M3s in cultures treated with 20gmg/ml of the various chemicals tested varied from 0% (Phosdrin) to 7% (parathion) as compared to 17% in control cultures. 11 out of the 14 organophosphates tested, significantly increased the SCE frequency. Of the 9 chemicals tested after metabolic activation by liver microsomal S9 preparation, significant increases in SCE frequency were seen in diazinon-, dimethoate-, Dursban- and Phosdrin-treated cells.
AB - In the present study, cytotoxic, cytostatic and cytogenetic effects of a number of organophosphate pesticides on human lymphoid cells (LAZ-007) in culture have been examined. Cytotoxic effects were dose related and often led to extensive cell kill. The pronounced effects of various organophosphates on the cell-cycle traverse were shown in data based on the enumeration of M1 and M3 metaphases after incubation of cells with BrdU. In cells incubated with 20 μg/ml of the various chemicals, the number of M1 metaphases ranged from 6% (R-1303) to 18% (Azodrin) compared to no M1 metaphases in control cultures. The number of M3s in cultures treated with 20gmg/ml of the various chemicals tested varied from 0% (Phosdrin) to 7% (parathion) as compared to 17% in control cultures. 11 out of the 14 organophosphates tested, significantly increased the SCE frequency. Of the 9 chemicals tested after metabolic activation by liver microsomal S9 preparation, significant increases in SCE frequency were seen in diazinon-, dimethoate-, Dursban- and Phosdrin-treated cells.
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U2 - 10.1016/0165-1218(82)90149-5
DO - 10.1016/0165-1218(82)90149-5
M3 - Article
C2 - 6981766
AN - SCOPUS:0019986129
VL - 102
SP - 89
EP - 102
JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
SN - 0165-1218
IS - 1
ER -