Cytokinetic and cytogenetic effect of agricultural chemicals on human lymphoid cells in vitro - II. Organochlorine pesticides

R. C. Sobti; A. Krishan; J. Davies

doi:10.1007/BF00333901

Cytokinetic and cytogenetic effect of agricultural chemicals on human lymphoid cells in vitro - II. Organochlorine pesticides

R. C. Sobti, A. Krishan, J. Davies

Pathology

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Human lymphoid cells of LAZ-007 cell line, incubated with 10^-4 to 10^-6 molar of eight different organochlorine pesticides had dose related cytotoxicity, mitotic depression and cell cycle traverse inhibition. In cultures incubated with 10^-4 M concentrations, M₁ metaphases were as high as 13% (Dicofol) as compared to less than 1% in the controls. The frequency of M₃ metaphases in cultures incubated with 10^-6 M concentrations ranged from 11% (Chlordane) to 15% (Endosulfan) compared to 17% in control cultures. Statistically significant increase in SCE frequency was seen in cells exposed to Chlordane, Dicofol, Endosulfan and Toxaphene. On metabolic activation with rat liver microsomal S-9 enzymes, Chlordane, Dicofol and Tetradifon induced SCE frequency was higher than that of nonactivated cultures.

Original language	English (US)
Pages (from-to)	221-231
Number of pages	11
Journal	Archives of Toxicology
Volume	52
Issue number	3
DOIs	https://doi.org/10.1007/BF00333901
State	Published - Mar 1983

Keywords

Chromosome replication
Organochlorine pesticides
Sister chromatid exchanges

ASJC Scopus subject areas

Toxicology
Health, Toxicology and Mutagenesis

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10.1007/BF00333901

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title = "Cytokinetic and cytogenetic effect of agricultural chemicals on human lymphoid cells in vitro - II. Organochlorine pesticides",

abstract = "Human lymphoid cells of LAZ-007 cell line, incubated with 10-4 to 10-6 molar of eight different organochlorine pesticides had dose related cytotoxicity, mitotic depression and cell cycle traverse inhibition. In cultures incubated with 10-4 M concentrations, M1 metaphases were as high as 13% (Dicofol) as compared to less than 1% in the controls. The frequency of M3 metaphases in cultures incubated with 10-6 M concentrations ranged from 11% (Chlordane) to 15% (Endosulfan) compared to 17% in control cultures. Statistically significant increase in SCE frequency was seen in cells exposed to Chlordane, Dicofol, Endosulfan and Toxaphene. On metabolic activation with rat liver microsomal S-9 enzymes, Chlordane, Dicofol and Tetradifon induced SCE frequency was higher than that of nonactivated cultures.",

keywords = "Chromosome replication, Organochlorine pesticides, Sister chromatid exchanges",

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AU - Sobti, R. C.

AU - Krishan, A.

AU - Davies, J.

PY - 1983/3

Y1 - 1983/3

N2 - Human lymphoid cells of LAZ-007 cell line, incubated with 10-4 to 10-6 molar of eight different organochlorine pesticides had dose related cytotoxicity, mitotic depression and cell cycle traverse inhibition. In cultures incubated with 10-4 M concentrations, M1 metaphases were as high as 13% (Dicofol) as compared to less than 1% in the controls. The frequency of M3 metaphases in cultures incubated with 10-6 M concentrations ranged from 11% (Chlordane) to 15% (Endosulfan) compared to 17% in control cultures. Statistically significant increase in SCE frequency was seen in cells exposed to Chlordane, Dicofol, Endosulfan and Toxaphene. On metabolic activation with rat liver microsomal S-9 enzymes, Chlordane, Dicofol and Tetradifon induced SCE frequency was higher than that of nonactivated cultures.

AB - Human lymphoid cells of LAZ-007 cell line, incubated with 10-4 to 10-6 molar of eight different organochlorine pesticides had dose related cytotoxicity, mitotic depression and cell cycle traverse inhibition. In cultures incubated with 10-4 M concentrations, M1 metaphases were as high as 13% (Dicofol) as compared to less than 1% in the controls. The frequency of M3 metaphases in cultures incubated with 10-6 M concentrations ranged from 11% (Chlordane) to 15% (Endosulfan) compared to 17% in control cultures. Statistically significant increase in SCE frequency was seen in cells exposed to Chlordane, Dicofol, Endosulfan and Toxaphene. On metabolic activation with rat liver microsomal S-9 enzymes, Chlordane, Dicofol and Tetradifon induced SCE frequency was higher than that of nonactivated cultures.

KW - Chromosome replication

KW - Organochlorine pesticides

KW - Sister chromatid exchanges

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Cytokinetic and cytogenetic effect of agricultural chemicals on human lymphoid cells in vitro - II. Organochlorine pesticides

Abstract

Keywords

ASJC Scopus subject areas

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