Cytokinetic and cytogenetic effect of agricultural chemicals on human lymphoid cells in vitro - II. Organochlorine pesticides

R. C. Sobti, A. Krishan, J. Davies

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Human lymphoid cells of LAZ-007 cell line, incubated with 10-4 to 10-6 molar of eight different organochlorine pesticides had dose related cytotoxicity, mitotic depression and cell cycle traverse inhibition. In cultures incubated with 10-4 M concentrations, M1 metaphases were as high as 13% (Dicofol) as compared to less than 1% in the controls. The frequency of M3 metaphases in cultures incubated with 10-6 M concentrations ranged from 11% (Chlordane) to 15% (Endosulfan) compared to 17% in control cultures. Statistically significant increase in SCE frequency was seen in cells exposed to Chlordane, Dicofol, Endosulfan and Toxaphene. On metabolic activation with rat liver microsomal S-9 enzymes, Chlordane, Dicofol and Tetradifon induced SCE frequency was higher than that of nonactivated cultures.

Original languageEnglish (US)
Pages (from-to)221-231
Number of pages11
JournalArchives of Toxicology
Volume52
Issue number3
DOIs
StatePublished - Mar 1983

Keywords

  • Chromosome replication
  • Organochlorine pesticides
  • Sister chromatid exchanges

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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