Cytokine pattern in peripheral blood mononuclear cells (PBMC) was studied in relation to disease progression in 62 HIV-infected children. Constitutive cytokine mRNA expression and phytohemagglutinin (PHA) or Staphylococcus aureus Cowan strain-1 (SAC)-induced protein levels of different cytokines were examined using reverse transcribed-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA) respectively. Constitutive mRNA expression of TNF-a, IFN-y and IL-10 was increased, with decreased IL-12 and undetectable IL-2 and IL-4. Stimulus-induced TNF-a, IFN-y, IL-12 and IL-4 secretion were impaired while IL-10 was unaffected. These patterns were exaggerated with declining immune status and/or rapid disease progression. TNF-a mRNA correlated also with plasma levels of HIV RNA. Thus, the constitutive cytokine response pattern in PBMC may differ from the stimulus-induced response. While type 1 cytokines are often impaired, increases in type 2 cytokines are rare. A dominant constitutive TNF-a response is indicative of a high virus load, immune suppression and disease progression.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology