Cytokine pattern in relation to disease progression in hivinfected children

S. Thanf, R. Hu, N. Ovaizu, J. Romano, X. P. Wangr, S. Pahwa

Research output: Contribution to journalArticle

Abstract

Cytokine pattern in peripheral blood mononuclear cells (PBMC) was studied in relation to disease progression in 62 HIV-infected children. Constitutive cytokine mRNA expression and phytohemagglutinin (PHA) or Staphylococcus aureus Cowan strain-1 (SAC)-induced protein levels of different cytokines were examined using reverse transcribed-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA) respectively. Constitutive mRNA expression of TNF-a, IFN-y and IL-10 was increased, with decreased IL-12 and undetectable IL-2 and IL-4. Stimulus-induced TNF-a, IFN-y, IL-12 and IL-4 secretion were impaired while IL-10 was unaffected. These patterns were exaggerated with declining immune status and/or rapid disease progression. TNF-a mRNA correlated also with plasma levels of HIV RNA. Thus, the constitutive cytokine response pattern in PBMC may differ from the stimulus-induced response. While type 1 cytokines are often impaired, increases in type 2 cytokines are rare. A dominant constitutive TNF-a response is indicative of a high virus load, immune suppression and disease progression.

Original languageEnglish (US)
Pages (from-to)A1221
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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  • Cite this

    Thanf, S., Hu, R., Ovaizu, N., Romano, J., Wangr, X. P., & Pahwa, S. (1996). Cytokine pattern in relation to disease progression in hivinfected children. FASEB Journal, 10(6), A1221.