Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue

Gordon Broderick, Ben Z. Katz, Henrique Fernandes, Mary A. Fletcher, Nancy Klimas, Frederick A. Smith, Maurice R G O'Gorman, Suzanne D. Vernon, Renee Taylor

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: As Chronic Fatigue Syndrome (CFS) has been known to follow Epstein-Bar virus (EBV) and other systemic infections; our objective was to describe differences in immune activation in post-infective CFS (PI-CFS) patients and recovered controls. We studied 301 adolescents prospectively over 24 months following the diagnosis of monospot-positive infectious mononucleosis (IM). We found an incidence of CFS at 6, 12 and 24 months of 13%, 7% and 4% respectively.Methods: Using chemiluminescent imaging we measured the concentrations of IL-1a, 1b, 2, 4, 5, 6, 8, 10, 12 (p70), 13, 15, 17 and 23, IFN-γ, TNF-α and TNF-β in duplicate plasma samples available in bio-bank from 9 PI-CFS subjects and 12 recovered controls at 24 months post-infection.Results: Standard comparative analysis indicated significant differences in IL-8 and 23 across subject groups. In constructing a linear classification model IL-6, 8 and 23 were selected by two different statistical approaches as discriminating features, with IL-1a, IL-2 and IFN-γ also selected in one model or the other. This supported an assignment accuracy of better than 80% at a confidence level of 0.95 into PI-CFS versus recovered controls.Conclusion: These results suggest that co-expression patterns in as few as 5 cytokines associated with Th17 function may hold promise as a tool for the diagnosis of post-infectious CFS.

Original languageEnglish
Article number191
JournalJournal of Translational Medicine
Volume10
Issue number1
DOIs
StatePublished - Sep 13 2012
Externally publishedYes

Fingerprint

Chronic Fatigue Syndrome
Fatigue
Fatigue of materials
Cytokines
Interleukin-23
Interleukin-8
Infectious Mononucleosis
Infection
Viruses
Interleukin-2
Linear Models
Interleukin-6
Chemical activation
Plasmas
Imaging techniques
Incidence

Keywords

  • Chronic fatigue
  • Classification model
  • Cytokines
  • Infectious mononucleosis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Broderick, G., Katz, B. Z., Fernandes, H., Fletcher, M. A., Klimas, N., Smith, F. A., ... Taylor, R. (2012). Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue. Journal of Translational Medicine, 10(1), [191]. https://doi.org/10.1186/1479-5876-10-191

Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue. / Broderick, Gordon; Katz, Ben Z.; Fernandes, Henrique; Fletcher, Mary A.; Klimas, Nancy; Smith, Frederick A.; O'Gorman, Maurice R G; Vernon, Suzanne D.; Taylor, Renee.

In: Journal of Translational Medicine, Vol. 10, No. 1, 191, 13.09.2012.

Research output: Contribution to journalArticle

Broderick, G, Katz, BZ, Fernandes, H, Fletcher, MA, Klimas, N, Smith, FA, O'Gorman, MRG, Vernon, SD & Taylor, R 2012, 'Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue', Journal of Translational Medicine, vol. 10, no. 1, 191. https://doi.org/10.1186/1479-5876-10-191
Broderick, Gordon ; Katz, Ben Z. ; Fernandes, Henrique ; Fletcher, Mary A. ; Klimas, Nancy ; Smith, Frederick A. ; O'Gorman, Maurice R G ; Vernon, Suzanne D. ; Taylor, Renee. / Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue. In: Journal of Translational Medicine. 2012 ; Vol. 10, No. 1.
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