Cytokine expression profiles of immune imbalance in post-mononucleosis chronic fatigue

Gordon Broderick, Ben Z. Katz, Henrique Fernandes, Mary A. Fletcher, Nancy Klimas, Frederick A. Smith, Maurice R.G. O'Gorman, Suzanne D. Vernon, Renee Taylor

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Background: As Chronic Fatigue Syndrome (CFS) has been known to follow Epstein-Bar virus (EBV) and other systemic infections; our objective was to describe differences in immune activation in post-infective CFS (PI-CFS) patients and recovered controls. We studied 301 adolescents prospectively over 24 months following the diagnosis of monospot-positive infectious mononucleosis (IM). We found an incidence of CFS at 6, 12 and 24 months of 13%, 7% and 4% respectively.Methods: Using chemiluminescent imaging we measured the concentrations of IL-1a, 1b, 2, 4, 5, 6, 8, 10, 12 (p70), 13, 15, 17 and 23, IFN-γ, TNF-α and TNF-β in duplicate plasma samples available in bio-bank from 9 PI-CFS subjects and 12 recovered controls at 24 months post-infection.Results: Standard comparative analysis indicated significant differences in IL-8 and 23 across subject groups. In constructing a linear classification model IL-6, 8 and 23 were selected by two different statistical approaches as discriminating features, with IL-1a, IL-2 and IFN-γ also selected in one model or the other. This supported an assignment accuracy of better than 80% at a confidence level of 0.95 into PI-CFS versus recovered controls.Conclusion: These results suggest that co-expression patterns in as few as 5 cytokines associated with Th17 function may hold promise as a tool for the diagnosis of post-infectious CFS.

Original languageEnglish (US)
Article number191
JournalJournal of translational medicine
Volume10
Issue number1
DOIs
StatePublished - Sep 13 2012

Keywords

  • Chronic fatigue
  • Classification model
  • Cytokines
  • Infectious mononucleosis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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