Cytogenetic analysis of Q-banded pronuclear chromosomes in fertilized Syrian hamster eggs

R. H. Martin, Wayne E Balkan, K. Burns

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The frequency and type of chromosome abnormalities were analyzed in 917 female pronuclei in Syrian hamster eggs fertilized by human sperm. Analysis at this stage allows detection of errors which have occurred during meiosis I and II. The chromosomes were Q-banded to identify individual chromosomes and detect subtle alterations. Thirty-three (3.6%) of the hamster egg complements were abnormal: 19 (2.1%) were hypohaploid, seven (0.76%) were hyperhaploid, two (0.2%) had double aneuploidy, and five (0.5%) had a structural chromosome abnormality. Since there were significantly more hypohaploid than hyperhaploid complements, a conservative estimate of aneuploidy can be derived by doubling the frequency of hyperhaploid complements. Thus a minimal estimate of aneuploidy (single, 1.5%, and double, 0.2%) is 1.7% and a minimal estimate of the total frequency of abnormalities is 2.2%. All chromosome groups were represented among the aneuploid complements suggesting that all chromosomes are susceptible to non-disjunction.

Original languageEnglish (US)
Pages (from-to)41-45
Number of pages5
JournalCytogenetics and Cell Genetics
Volume35
Issue number1
StatePublished - 1983
Externally publishedYes

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Cytogenetic Analysis
Mesocricetus
Aneuploidy
Eggs
Chromosomes
Chromosome Aberrations
Zygote
Meiosis
Cricetinae
Ovum
Spermatozoa

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

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Cytogenetic analysis of Q-banded pronuclear chromosomes in fertilized Syrian hamster eggs. / Martin, R. H.; Balkan, Wayne E; Burns, K.

In: Cytogenetics and Cell Genetics, Vol. 35, No. 1, 1983, p. 41-45.

Research output: Contribution to journalArticle

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