Cytogenetic analysis of Q-banded pronuclear chromosomes in fertilized Syrian hamster eggs

R. H. Martin, W. Balkan, K. Burns

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The frequency and type of chromosome abnormalities were analyzed in 917 female pronuclei in Syrian hamster eggs fertilized by human sperm. Analysis at this stage allows detection of errors which have occurred during meiosis I and II. The chromosomes were Q-banded to identify individual chromosomes and detect subtle alterations. Thirty-three (3.6%) of the hamster egg complements were abnormal: 19 (2.1%) were hypohaploid, seven (0.76%) were hyperhaploid, two (0.2%) had double aneuploidy, and five (0.5%) had a structural chromosome abnormality. Since there were significantly more hypohaploid than hyperhaploid complements, a conservative estimate of aneuploidy can be derived by doubling the frequency of hyperhaploid complements. Thus a minimal estimate of aneuploidy (single, 1.5%, and double, 0.2%) is 1.7% and a minimal estimate of the total frequency of abnormalities is 2.2%. All chromosome groups were represented among the aneuploid complements suggesting that all chromosomes are susceptible to non-disjunction.

Original languageEnglish (US)
Pages (from-to)41-45
Number of pages5
JournalCytogenetics and cell genetics
Volume35
Issue number1
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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