Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae

Paula Ludovico, Fernando Rodrigues, Agostinho Almeida, Manuel T. Silva, Antonio Barrientos, Manuela Côrte-Real

Research output: Contribution to journalArticle

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Abstract

Evidence is presented that mitochondria are implicated in the previously described programmed cell death (PCD) process induced by acetic acid in Saccharomyces cerevisiae. In yeast cells undergoing a PCD process induced by acetic acid, translocation of cytochrome c (CytC) to the cytosol and reactive oxygen species production, two events known to be proapoptotic in mammals, were observed. Associated with these events, reduction in oxygen consumption and in mitochondrial membrane potential was found. Enzymatic assays showed that the activity of complex bc1 was normal, whereas that of cytochrome c oxidase (COX) was strongly decreased. This decrease is in accordance with the observed reduction in the amounts of COX II subunit and of cytochromes a+a3. The acetic acid-induced PCD process was found to be independent of oxidative phosphorylation because it was not inhibited by oligomycin treatment. The inability of S. cerevisiae mutant strains (lacking mitochondrial DNA, heme lyase, or ATPase) to undergo acetic acid-induced PCD and in the ATPase mutant (knockout in ATP10) the absence of CytC release provides further evidence that the process is mediated by a mitochondria-dependent apoptotic pathway. The understanding of the involvement of a mitochondria-dependent apoptotic pathway in S. cerevisiae PCD process will be most useful in the further elucidation of an ancestral pathway common to PCD in metazoans.

Original languageEnglish
Pages (from-to)2598-2606
Number of pages9
JournalMolecular Biology of the Cell
Volume13
Issue number8
DOIs
StatePublished - Aug 1 2002
Externally publishedYes

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Cytochromes c
Acetic Acid
Saccharomyces cerevisiae
Mitochondria
Cell Death
Electron Transport Complex IV
Adenosine Triphosphatases
Oligomycins
Lyases
Mitochondrial Membrane Potential
Oxidative Phosphorylation
Enzyme Assays
Heme
Mitochondrial DNA
Oxygen Consumption
Cytosol
Mammals
Reactive Oxygen Species
Oxidoreductases
Yeasts

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae. / Ludovico, Paula; Rodrigues, Fernando; Almeida, Agostinho; Silva, Manuel T.; Barrientos, Antonio; Côrte-Real, Manuela.

In: Molecular Biology of the Cell, Vol. 13, No. 8, 01.08.2002, p. 2598-2606.

Research output: Contribution to journalArticle

Ludovico, Paula ; Rodrigues, Fernando ; Almeida, Agostinho ; Silva, Manuel T. ; Barrientos, Antonio ; Côrte-Real, Manuela. / Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 8. pp. 2598-2606.
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