Cytochemical characteristics of cat spinal neurons activated during fictive locomotion

P. A. Carr, A. Huang, B. R. Noga, L. M. Jordan

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Using standard immunohistochemical and histochemical techniques, we have examined the neurochemical characteristics of a subpopulation of locomotor-related neurons as labeled by the activity-dependent marker c-fos. Results were compared to those obtained from a small sample of intracellulariy labeled locomotor-related neurons. In the paralyzed, decerebrate cat, fictive locomotion was evoked by electrical stimulation of the mesencephalic locomotor region. Most c-fos-immunoreactive neurons were distributed in medial lamina VI and VII and in lamina VIII and X. Double labeling of c-fos with various cytochemical markers revealed that about one-third of the c-fos-immunoreactive neurons were choline acetyltransferase immtmoroactive, about one-third were glutamate immunoreactive, and about one-third were aspartate immunoreactive. In addition, approximately 15% of the c-fos-labeled neurons contained NADPH-diaphorase reaction product, while almost 40% appeared to receive close contacts from calcitonin generelated peptide-immunoreactive fibers and boutons. Choline acetyfransferase- or aspartate immunoreactivity was observed in some intracellulariy, labeled neurons. These findings have implications regarding the putative neurotransmitters utilized by subpopulations of locomotor-related neurons in the cat spinal cord.

Original languageEnglish (US)
Pages (from-to)213-218
Number of pages6
JournalBrain Research Bulletin
Volume37
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • Aspertate
  • c-fos
  • Calcitonin gene-related peptide
  • Choline acetyitransferase
  • Glutamate
  • Immunohistochemistry
  • NADPHdiaphorase
  • Spinal cord
  • Tetramethy1lrhodamine-dextran

ASJC Scopus subject areas

  • Neuroscience(all)

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