Abstract
Using standard immunohistochemical and histochemical techniques, we have examined the neurochemical characteristics of a subpopulation of locomotor-related neurons as labeled by the activity-dependent marker c-fos. Results were compared to those obtained from a small sample of intracellulariy labeled locomotor-related neurons. In the paralyzed, decerebrate cat, fictive locomotion was evoked by electrical stimulation of the mesencephalic locomotor region. Most c-fos-immunoreactive neurons were distributed in medial lamina VI and VII and in lamina VIII and X. Double labeling of c-fos with various cytochemical markers revealed that about one-third of the c-fos-immunoreactive neurons were choline acetyltransferase immtmoroactive, about one-third were glutamate immunoreactive, and about one-third were aspartate immunoreactive. In addition, approximately 15% of the c-fos-labeled neurons contained NADPH-diaphorase reaction product, while almost 40% appeared to receive close contacts from calcitonin generelated peptide-immunoreactive fibers and boutons. Choline acetyfransferase- or aspartate immunoreactivity was observed in some intracellulariy, labeled neurons. These findings have implications regarding the putative neurotransmitters utilized by subpopulations of locomotor-related neurons in the cat spinal cord.
Original language | English (US) |
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Pages (from-to) | 213-218 |
Number of pages | 6 |
Journal | Brain Research Bulletin |
Volume | 37 |
Issue number | 2 |
DOIs | |
State | Published - 1995 |
Externally published | Yes |
Keywords
- Aspertate
- c-fos
- Calcitonin gene-related peptide
- Choline acetyitransferase
- Glutamate
- Immunohistochemistry
- NADPHdiaphorase
- Spinal cord
- Tetramethy1lrhodamine-dextran
ASJC Scopus subject areas
- Neuroscience(all)