Cytochalasin B induces cellular DNA fragmentation

M. A. Kolber, K. O. Broschat, B. Landa-Gonzalez

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

Cellular DNA fragmentation can be induced in many biological instances without plasma membrane damage. The fungal metabolite, cytochalasin B, is capable of modifying numerous cellular functions related to DNA synthesis. In this work it is demonstrated that cytochalasin B is capable of inducing DNA fragmentation in a number of cells lines. This DNA fragmentation occurs before plasma membrane lysis and over a period of hours. Cytochalasin E and villin, agents that act on the microfilaments, also induce DNA fragmentation. Phorbol dibutyrate, a diacylglyceral analog, is able to inhibit cytochalasin B-induced DNA fragmentation in a dosedependent fashion. These findings support the interpretation that cytochalasin B is inducing DNA fragmentation via its effect on the actin filaments.

Original languageEnglish (US)
Pages (from-to)3021-3027
Number of pages7
JournalFASEB Journal
Volume4
Issue number12
DOIs
StatePublished - Jan 1 1990

Keywords

  • Actin
  • Cytochalasin
  • DNA fragmentation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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