Cytochalasin B induces cellular DNA fragmentation

Michael A Kolber, Kay O. Broschat, Belkis Landa-Gonzalez

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

Cellular DNA fragmentation can be induced in many biological instances without plasma membrane damage. The fungal metabolite, cytochalasin B, is capable of modifying numerous cellular functions related to DNA synthesis. In this work it is demonstrated that cytochalasin B is capable of inducing DNA fragmentation in a number of cells lines. This DNA fragmentation occurs before plasma membrane lysis and over a period of hours. Cytochalasin E and villin, agents that act on the microfilaments, also induce DNA fragmentation. Phorbol dibutyrate, a diacylglyceral analog, is able to inhibit cytochalasin B-induced DNA fragmentation in a dosedependent fashion. These findings support the interpretation that cytochalasin B is inducing DNA fragmentation via its effect on the actin filaments.

Original languageEnglish
Pages (from-to)3021-3027
Number of pages7
JournalFASEB Journal
Volume4
Issue number12
StatePublished - Dec 1 1990

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Keywords

  • Actin
  • Cytochalasin
  • DNA fragmentation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Kolber, M. A., Broschat, K. O., & Landa-Gonzalez, B. (1990). Cytochalasin B induces cellular DNA fragmentation. FASEB Journal, 4(12), 3021-3027.