Cysteamine selectively enhances neuropeptide Y2 receptor binding activity

Wei Li, Terry D. Hexum

Research output: Contribution to journalArticle

Abstract

Affinity labeling of [125I]NPY to the bovine hippocampal NPY receptor has revealed a 50 kDa specific binding protein, the Y2 receptor. Cysteamine (10 μM - 10 mM) specifically enhanced NPY specific labeling of the Y2 receptor without affecting crosslinking effeiciency. Several structurally related agents, including reduced glutathione, cysteine, β-mercaptoethanol and ethanolamine, were without effect on receptor binding. The enhancement of binding by cysteamine could be reversed by washing the membranes. These studies suggest that cysteamine may change the conformation of the NPY Y2 receptor and increase its binding activity.

Original languageEnglish (US)
Pages (from-to)380-386
Number of pages7
JournalBiochemical and biophysical research communications
Volume184
Issue number1
DOIs
StatePublished - Apr 15 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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