Cysteamine selectively enhances neuropeptide Y2 receptor binding activity

Wei Li; Terry D. Hexum

doi:10.1016/0006-291X(92)91204-4

Cysteamine selectively enhances neuropeptide Y₂ receptor binding activity

Wei Li, Terry D. Hexum

Research output: Contribution to journal › Article

Abstract

Affinity labeling of [¹²⁵I]NPY to the bovine hippocampal NPY receptor has revealed a 50 kDa specific binding protein, the Y₂ receptor. Cysteamine (10 μM - 10 mM) specifically enhanced NPY specific labeling of the Y₂ receptor without affecting crosslinking effeiciency. Several structurally related agents, including reduced glutathione, cysteine, β-mercaptoethanol and ethanolamine, were without effect on receptor binding. The enhancement of binding by cysteamine could be reversed by washing the membranes. These studies suggest that cysteamine may change the conformation of the NPY Y₂ receptor and increase its binding activity.

Original language	English (US)
Pages (from-to)	380-386
Number of pages	7
Journal	Biochemical and biophysical research communications
Volume	184
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(92)91204-4
State	Published - Apr 15 1992
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Li, W., & Hexum, T. D. (1992). Cysteamine selectively enhances neuropeptide Y₂ receptor binding activity. Biochemical and biophysical research communications, 184(1), 380-386. https://doi.org/10.1016/0006-291X(92)91204-4

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